Home LiteratureArticle Details
PMID: 16043509 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p.

RNA (New York, N.Y.) ·Vol. 11 ·No. 8 ·2005-08-00 ·Pages 1291-302

Wang L, Lewis MS, Johnson AW

Abstract

The Ski complex (composed of Ski3p, Ski8p, and the DEVH ATPase Ski2p) is a central component of the 3'-5' cytoplasmic mRNA degradation pathway in yeast. Although the proteins of the complex interact with each other as well as with Ski7p to mediate degradation by exosome, a 3'-exonuclease complex, the nature of these interactions is not well understood. Here we explore interactions within the Ski complex and between the Ski complex and Ski7p using a directed two-hybrid approach combined with coimmunoprecipitation experiments. We also test the functional significance of these interactions in vivo. Our results suggest that within the Ski complex, Ski3p serves as a scaffold protein with its C terminus interacting with Ski8p, and the sub-C terminus interacting with Ski2p, while no direct interaction between Ski2p and Ski8p was found. Ski7p interacts with the Ski complex via its interaction with Ski8p and Ski3p. In addition, inactivating the Ski complex by mutating conserved residues in the DEVH helicase motif of Ski2 did not abrogate its interaction with Ski7p, indicating that Ski2p function is not necessary for this interaction.

MeSH Terms
Adaptor Proteins, Signal Transducing Binding Sites Kinetics Macromolecular Substances/chemistry,metabolism Nuclear Proteins/chemistry,metabolism Protein Conformation Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/chemistry,metabolism
Chemicals
Adaptor Proteins, Signal Transducing Macromolecular Substances Nuclear Proteins SKI2 protein, S cerevisiae SKI3 protein, S cerevisiae SKI7 protein, S cerevisiae SKI8 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang Lingna
Section of Molecular Genetics and Microbiology, The University of Texas, Austin, TX 78712-0162, USA.
Lewis Marc S
Johnson Arlen W
References (35)
35 references, click to expand
  1. Chromosomal superkiller mutants of Saccharomyces cerevisiae.
    J Bacteriol. 1978 Dec;136(3):1002-7 PMID: 363683
  2. Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
    Protein Sci. 2004 Oct;13(10):2673-84 PMID: 15340168
  3. Structure and nuclear localization signal of the SKI3 antiviral protein of Saccharomyces cerevisiae.
    Yeast. 1989 May-Jun;5(3):149-58 PMID: 2660461
  4. The TPR snap helix: a novel protein repeat motif from mitosis to transcription.
    Trends Biochem Sci. 1991 May;16(5):173-7 PMID: 1882418
  5. A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins.
    Yeast. 1993 Jan;9(1):43-51 PMID: 8442386
  6. Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA.
    Mol Cell Biol. 1993 Jul;13(7):4331-41 PMID: 8321235
  7. Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.
    Mol Cell Biol. 1993 Aug;13(8):4826-35 PMID: 8336719
  8. Premature translational termination triggers mRNA decapping.
    Nature. 1994 Aug 18;370(6490):578-81 PMID: 8052314
  9. Mechanisms of mRNA degradation in eukaryotes.
    Trends Biochem Sci. 1994 Aug;19(8):336-40 PMID: 7940679
  10. Degradation of mRNA in eukaryotes.
    Cell. 1995 Apr 21;81(2):179-83 PMID: 7736570
  11. Synthetic lethality of sep1 (xrn1) ski2 and sep1 (xrn1) ski3 mutants of Saccharomyces cerevisiae is independent of killer virus and suggests a general role for these genes in translation control.
    Mol Cell Biol. 1995 May;15(5):2719-27 PMID: 7739552
  12. RNA turnover: the helicase story unwinds.
    Curr Biol. 1996 Jul 1;6(7):780-2 PMID: 8805288
  13. Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.
    Microbiol Rev. 1996 Mar;60(1):233-49 PMID: 8852902
  14. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast.
    Genetics. 1996 Dec;144(4):1425-36 PMID: 8978031
  15. The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
    EMBO J. 1998 Mar 2;17(5):1497-506 PMID: 9482746
  16. Ski6p is a homolog of RNA-processing enzymes that affects translation of non-poly(A) mRNAs and 60S ribosomal subunit biogenesis.
    Mol Cell Biol. 1998 May;18(5):2688-96 PMID: 9566888
  17. Mutational analysis of exoribonuclease I from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1998 Aug 15;26(16):3707-16 PMID: 9685486
  18. NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae.
    Mol Cell Biol. 1999 Mar;19(3):2389-99 PMID: 10022925
  19. The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae.
    J Virol. 1999 Apr;73(4):2893-900 PMID: 10074137
  20. Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families.
    Trends Biochem Sci. 1999 May;24(5):192-8 PMID: 10322435
  21. Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs.
    Mol Cell Biol. 2000 Jan;20(2):441-52 PMID: 10611222
  22. The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.
    RNA. 2000 Mar;6(3):449-57 PMID: 10744028
  23. mRNA stability in eukaryotes.
    Curr Opin Genet Dev. 2000 Apr;10(2):193-8 PMID: 10753781
  24. Musing on the structural organization of the exosome complex.
    Nat Struct Biol. 2000 Oct;7(10):843-6 PMID: 11017189
  25. Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast.
    EMBO J. 2001 Sep 3;20(17):4684-93 PMID: 11532933
  26. A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant.
    RNA. 2001 Nov;7(11):1566-77 PMID: 11720286
  27. Functional link between the mammalian exosome and mRNA decapping.
    Cell. 2001 Dec 14;107(6):751-62 PMID: 11747811
  28. The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements.
    EMBO J. 2002 Jan 15;21(1-2):165-74 PMID: 11782436
  29. An mRNA surveillance mechanism that eliminates transcripts lacking termination codons.
    Science. 2002 Mar 22;295(5563):2258-61 PMID: 11910109
  30. Exosome-mediated recognition and degradation of mRNAs lacking a termination codon.
    Science. 2002 Mar 22;295(5563):2262-4 PMID: 11910110
  31. An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation.
    Mol Cell. 2003 May;11(5):1405-13 PMID: 12769863
  32. Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast.
    EMBO J. 2003 Aug 1;22(15):3951-9 PMID: 12881429
  33. Structure-based assembly of protein complexes in yeast.
    Science. 2004 Mar 26;303(5666):2026-9 PMID: 15044803
  34. The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure.
    Protein Sci. 2004 Jun;13(6):1557-65 PMID: 15152089
  35. Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN.
    Mol Cell Biol. 1984 Apr;4(4):761-70 PMID: 6371496
Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.2060405
Published
2005-08-00
Pages
1291-302
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370812
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.2060405 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2