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PMID: 16217606 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Reverse genetic approaches for functional genomics of rice.

Plant molecular biology ·Vol. 59 ·No. 1 ·2005-09-00 ·Pages 111-23

An G, Jeong DH, Jung KH, Lee S

Abstract

T-DNA and transposable elements e.g., Ds and Tos17, are used to generate a large number of insertional mutant lines in rice. Some carry the GUS or GFP reporter for gene trap or enhancer trap. These reporter systems are valuable for identifying tissue- or organ-preferential genes. Activation tagging lines have also been generated for screening mutants and isolating mutagenized genes. To utilize these resources more efficiently, tagged lines have been produced for reverse genetic approaches. DNA pools of the T-DNA tagged lines and Tos17 lines have been prepared for PCR screening of insertional mutants in a given gene. Tag end sequences (TES) of the inserts have also been produced. TES databases are beneficial for analyzing the function of a large number of rice genes.

MeSH Terms
Binding Sites/genetics DNA Transposable Elements/genetics DNA, Bacterial/genetics DNA, Plant/genetics Databases, Genetic Genes, Plant/genetics Genes, Reporter/genetics Genomics/methods Mutagenesis, Insertional/methods Mutation/genetics Oryza/genetics Retroelements/genetics
Chemicals
DNA Transposable Elements DNA, Bacterial DNA, Plant Retroelements T-DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
An Gynheung
National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea. [email protected]
Jeong Dong-Hoon
Jung Ki-Hong
Lee Sichul
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-004-4037-y
Email
[email protected]
Published
2005-09-00
Pages
111-23
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-004-4037-y View journal details
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