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PMID: 1655718 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Klebsiella aerogenes catabolite gene activator protein and the gene encoding it (crp).

Journal of bacteriology ·Vol. 173 ·No. 20 ·1991-10-00 ·Pages 6626-31

Osuna R, Bender RA

Abstract

The catabolite gene activator protein from Klebsiella aerogenes (CAPK) and the corresponding protein from Escherichia coli (CAPE) were shown to be nearly identical. Both CAPK and CAPE activated transcription from the CAP-dependent promoters derived from E. coli and K. aerogenes. The crp gene from K. aerogenes (encoding CAP) is tightly linked to rpsL. The nucleotide sequence of crp predicts an amino acid sequence for CAPK that differs in only one position from that of CAPE.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Binding Sites/genetics Carrier Proteins/genetics Cloning, Molecular Cyclic AMP Receptor Protein/genetics Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial/genetics Klebsiella pneumoniae/genetics Molecular Sequence Data Mutation/genetics Promoter Regions, Genetic/genetics Receptors, Cyclic AMP/genetics Ribosomal Protein S9 Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid beta-Galactosidase/genetics
Chemicals
Bacterial Proteins Carrier Proteins Cyclic AMP Receptor Protein Escherichia coli Proteins Receptors, Cyclic AMP Ribosomal Protein S9 RpsI protein, E coli beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Osuna R
Department of Biology, University of Michigan, Ann Arbor 48109-1048.
Bender R A
References (27)
27 references, click to expand
  1. In vitro transcription of the histidine utilization (hutUH) operon from Klebsiella aerogenes.
    J Bacteriol. 1991 Jan;173(1):116-23 PMID: 1846133
  2. Genetic control of glutamine synthetase in Klebiella aerogenes.
    J Bacteriol. 1975 Jan;121(1):320-31 PMID: 234939
  3. RNA polymerase as a repressor of transcription in the hut(P) region of mutant Klebsiella aerogenes histidine utilization operons.
    J Bacteriol. 1988 Oct;170(10):4986-90 PMID: 3170491
  4. Regulation of the galactose-inducible lac operon and the histidine utilization operons in pts mutants of Klebsiella aerogenes.
    J Bacteriol. 1988 Dec;170(12):5588-93 PMID: 3142852
  5. Bidirectional promoter in the hut(P) region of the histidine utilization (hut) operons from Klebsiella aerogenes.
    J Bacteriol. 1988 May;170(5):2240-6 PMID: 2834335
  6. Regulation of hutUH operon expression by the catabolite gene activator protein-cyclic AMP complex in Klebsiella aerogenes.
    J Bacteriol. 1984 Sep;159(3):934-9 PMID: 6090399
  7. crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli.
    J Bacteriol. 1986 Aug;167(2):639-46 PMID: 3525518
  8. Nucleotide sequence of Klebsiella pneumoniae lac genes.
    J Bacteriol. 1985 Sep;163(3):850-7 PMID: 3897196
  9. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  10. Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1594-8 PMID: 6300859
  11. CAP and RNA polymerase interactions with the lac promoter: binding stoichiometry and long range effects.
    Nucleic Acids Res. 1983 Jan 11;11(1):141-58 PMID: 6306561
  12. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
  13. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  14. Cloning and sequence of the crp gene of Escherichia coli K 12.
    Nucleic Acids Res. 1982 Feb 25;10(4):1363-78 PMID: 6280141
  15. Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein.
    Nucleic Acids Res. 1982 Feb 25;10(4):1345-61 PMID: 6280140
  16. Stoichiometry of catabolite activator protein/adenosine cyclic 3',5'-monophosphate interactions at the lac promoter of Escherichia coli.
    Biochemistry. 1982 Nov 23;21(24):6032-6 PMID: 6295452
  17. Mutations in the Escherichia coli operon that define two promoters and the binding site of the cyclic AMP receptor protein.
    J Mol Biol. 1982 Jan 15;154(2):211-27 PMID: 6804633
  18. A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1836-40 PMID: 325556
  19. Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1831-5 PMID: 325555
  20. Glutamine synthetase regulation, adenylylation state, and strain specificity analyzed by polyacrylamide gel electrophoresis.
    J Bacteriol. 1979 Feb;137(2):1000-7 PMID: 33958
  21. Biochemical parameters of glutamine synthetase from Klebsiella aerogenes.
    J Bacteriol. 1977 Feb;129(2):1001-9 PMID: 14104
  22. Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5090-4 PMID: 228278
  23. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants.
    J Bacteriol. 1978 Jan;133(1):149-57 PMID: 201606
  24. Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes.
    J Biol Chem. 1971 Oct 25;246(20):6288-96 PMID: 4331387
  25. Regulation of histidase synthesis in intergeneric hybrids of enteric bacteria.
    J Bacteriol. 1976 Jul;127(1):114-9 PMID: 6426
  26. Deletion of the Escherichia coli crp gene.
    J Bacteriol. 1975 Apr;122(1):338-40 PMID: 164435
  27. Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing.
    J Bacteriol. 1986 Oct;168(1):357-64 PMID: 3020001
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.20.6626-6631.1991
Published
1991-10-00
Pages
6626-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209001
Subset
IM
Grants
NIAID NIH HHS · AI15822 · United States
Databases
GENBANK
M63251, M63252, M63253, M63254, M63255, M68973, S59272, S59273, S59274, S59877, S59917
External Links
PubMed source DOI: 10.1128/jb.173.20.6626-6631.1991 PMC Full Text View journal details
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