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PMID: 3525518 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli.

Journal of bacteriology ·Vol. 167 ·No. 2 ·1986-08-00 ·Pages 639-46

Cossart P, Groisman EA, Serre MC, Casadaban MJ, Gicquel-Sanzey B

Abstract

The complete nucleotide sequences of the Salmonella typhimurium LT2 and Shigella flexneri 2B crp genes were determined and compared with those of the Escherichia coli K-12 crp gene. The Shigella flexneri gene was almost like the E. coli crp gene, with only four silent base pair changes. The S. typhimurium and E. coli crp genes presented a higher degree of divergence in their nucleotide sequence with 77 changes, but the corresponding amino acid sequences presented only one amino acid difference. The nucleotide sequences of the crp genes diverged to the same extent as in the other genes, trp, ompA, metJ, and araC, which are structural or regulatory genes. An analysis of the amino acid divergence, however, revealed that the catabolite gene activator protein, the crp gene product, is the most conserved protein observed so far. Comparison of codon usage in S. typhimurium and E. coli for all genes sequenced in both organisms showed that their patterns were similar. Comparison of the regulatory regions of the S. typhimurium and E. coli crp genes showed that the most conserved sequences were those known to be essential for the expression of E. coli crp.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon DNA, Bacterial/genetics Escherichia coli/genetics Genes Genes, Bacterial Genes, Regulator Receptors, Cyclic AMP/genetics Salmonella typhimurium/genetics Shigella flexneri/genetics
Chemicals
Codon DNA, Bacterial Receptors, Cyclic AMP
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cossart P
Groisman E A
Serre M C
Casadaban M J
Gicquel-Sanzey B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.2.639-646.1986
Published
1986-08-00
Pages
639-46
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212937
Subset
IM
Grants
PHS HHS · A 100468 · United States
NIGMS NIH HHS · GM 29.067 · United States
Databases
GENBANK
M13772, M13773
External Links
PubMed source DOI: 10.1128/jb.167.2.639-646.1986 PMC Full Text View journal details
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