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PMID: 1690847 Published · ppublish English Journal Article

Antisense-fos RNA causes partial reversion of the transformed phenotypes induced by the c-Ha-ras oncogene.

Molecular and cellular biology ·Vol. 10 ·No. 4 ·1990-04-00 ·Pages 1545-55

Ledwith BJ, Manam S, Kraynak AR, Nichols WW, Bradley MO

Abstract

Several lines of evidence have suggested that c-fos may act downstream from c-Ha-ras in a growth-regulatory signal transduction pathway. We used antisense RNA to inhibit c-fos gene expression and investigated the effects of diminished c-fos expression on the phenotypes induced by the EJ c-Ha-ras oncogene in NIH 3T3 cells. Immunofluorescent staining demonstrated that the antisense RNA caused a marked reduction in the amount of c-fos protein expressed following serum stimulation. EJ cells containing antisense-fos RNA continued to overexpress ras and remained capable of proliferating in vitro. However, the antisense-fos RNA caused a partial reversion of the major transformed phenotypes of EJ cells, including a restoration of both density-dependent growth arrest and the ability to be rendered quiescent by serum deprivation, a reversion to a flat morphology, inhibition of anchorage-independent growth, and inhibition of tumorigenicity in nude mice. Our results indicate that inhibition of c-fos expression, to a level still supporting in vitro proliferation, prevents the transforming effects of the ras oncogene; they thus provide additional evidence for the participation of c-fos in ras-regulated signal transduction pathways.

MeSH Terms
Animals Blotting, Southern Cell Division Cell Line Cell Transformation, Neoplastic Cells, Cultured DNA Replication DNA, Neoplasm/genetics Dexamethasone/pharmacology Female Gene Expression Regulation, Neoplastic Genes, ras Humans Kinetics Mice Mice, Nude Neoplasm Transplantation Phenotype Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Proto-Oncogenes RNA/genetics RNA, Antisense RNA, Messenger/antagonists & inhibitors Transfection Transplantation, Heterologous Urinary Bladder Neoplasms/genetics
Chemicals
DNA, Neoplasm Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos RNA, Antisense RNA, Messenger RNA Dexamethasone Protein-Tyrosine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ledwith B J
Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486.
Manam S
Kraynak A R
Nichols W W
Bradley M O
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47 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
1990-04-00
Pages
1545-55
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362259
Subset
IM
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