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PMID: 1847508 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 4 ·1991-02-15 ·Pages 1095-9

Keegan K, Johnson DE, Williams LT, Hayman MJ

Abstract

The fibroblast growth factors are a family of polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing. Fibroblast growth factor receptors (FGFRs) have previously been identified in chicken, mouse, and human and have been shown to contain an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. We have isolated a human cDNA for another tyrosine kinase receptor that is highly homologous to the previously described FGFR. Expression of this receptor cDNA in COS cells directs the expression of a 125-kDa glycoprotein. We demonstrate that this cDNA encodes a biologically active receptor by showing that human acidic and basic fibroblast growth factors activate this receptor as measured by 45Ca2+ efflux assays. These data establish the existence of an additional member of the FGFR family that we have named FGFR-3.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Calcium/metabolism Cell Line Cloning, Molecular Fibroblast Growth Factors/metabolism Gene Library Humans Molecular Sequence Data Multigene Family Oligonucleotide Probes RNA, Messenger/genetics,isolation & purification Receptors, Cell Surface/genetics,isolation & purification Receptors, Fibroblast Growth Factor Transfection
Chemicals
Oligonucleotide Probes RNA, Messenger Receptors, Cell Surface Receptors, Fibroblast Growth Factor Fibroblast Growth Factors Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Keegan K
Department of Microbiology, State University of New York, Stony Brook 11794.
Johnson D E
Williams L T
Hayman M J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.4.1095
Published
1991-02-15
Pages
1095-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50963
Subset
IM
Grants
NHLBI NIH HHS · P01 HL-43821 · United States
NCI NIH HHS · R01 CA-42573 · United States
NHLBI NIH HHS · R01 HL-32989 · United States
Databases
GENBANK
M58051
External Links
PubMed source DOI: 10.1073/pnas.88.4.1095 PMC Full Text View journal details
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