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PMID: 19226188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome-wide control of the distribution of meiotic recombination.

PLoS biology ·Vol. 7 ·No. 2 ·2009-02-17 ·Pages e35

Grey C, Baudat F, de Massy B

Abstract

Meiotic recombination events are not randomly distributed in the genome but occur in specific regions called recombination hotspots. Hotspots are predicted to be preferred sites for the initiation of meiotic recombination and their positions and activities are regulated by yet-unknown controls. The activity of the Psmb9 hotspot on mouse Chromosome 17 (Chr 17) varies according to genetic background. It is active in strains carrying a recombinant Chr 17 where the proximal third is derived from Mus musculus molossinus. We have identified the genetic locus required for Psmb9 activity, named Dsbc1 for Double-strand break control 1, and mapped this locus within a 6.7-Mb region on Chr 17. Based on cytological analysis of meiotic DNA double-strand breaks (DSB) and crossovers (COs), we show that Dsbc1 influences DSB and CO, not only at Psmb9, but in several other regions of Chr 17. We further show that CO distribution is also influenced by Dsbc1 on Chrs 15 and 18. Finally, we provide direct molecular evidence for the regulation in trans mediated by Dsbc1, by showing that it controls the CO activity at the Hlx1 hotspot on Chr 1. We thus propose that Dsbc1 encodes for a trans-acting factor involved in the specification of initiation sites of meiotic recombination genome wide in mice.

MeSH Terms
Animals Cell Nucleus/chemistry,metabolism Chromosome Mapping Chromosomes, Mammalian Crossing Over, Genetic Cysteine Endopeptidases/genetics DNA Breaks, Double-Stranded Genome Genome-Wide Association Study In Situ Hybridization, Fluorescence Male Meiosis/genetics Mice Mice, Inbred Strains/genetics Microsatellite Repeats Recombination, Genetic/genetics Spermatocytes/chemistry,cytology,metabolism
Chemicals
LMP-2 protein Cysteine Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grey Corinne
Institut de Génétique Humaine, UPR1142-CNRS, Montpellier, France.
Baudat Frédéric
de Massy Bernard
Conflict of Interest

Competing interests. The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.1000035
Published
2009-02-17
Pages
e35
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC2642883
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pbio.1000035 PMC Full Text View journal details
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