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PMID: 3023057 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over.

The EMBO journal ·Vol. 5 ·No. 9 ·1986-09-00 ·Pages 2123-9

Uematsu Y, Kiefer H, Schulze R, Fischer-Lindahl K, Steinmetz M

Abstract

We have cloned and sequenced a meiotic recombinational hotspot between the A beta 3 and A beta 2 genes in the major histocompatibility complex (MHC) of the mouse. This recombinational hotspot in the Mus musculus castaneus cas3 haplotype was previously localized to a region of 9.5 kb of DNA in which five independent crossing-over events occurred at the unusually high frequency of 0.6%. Aside from cas3, the hotspot appears to be absent in many other MHC haplotypes. We have now confined the five recombinational breakpoints to a stretch of 3.5 kb of DNA. From the nucleotide sequence around the recombinational breakpoints, determined in the parental cas3 and b haplotypes as well as for two recombinant haplotypes, we show that the two recombinant haplotypes were generated by homologous equal crossing-over and place the breakpoints within two non-overlapping stretches of 10 and 36 bp, respectively. Comparison of the DNA sequences of the hotspot-positive cas3 and the hotspot-negative b haplotypes reveals a number of differences, in particular, a CAGA-repeat sequence which is present in CAS3 in six, but only four copies in C57BL/6 DNA. This repeat sequence is reminiscent of one in a previously characterized hotspot in the E beta gene.

MeSH Terms
Animals Base Composition Base Sequence Crossing Over, Genetic DNA Restriction Enzymes DNA, Recombinant/metabolism Haplotypes Major Histocompatibility Complex Meiosis Mice Nucleic Acid Hybridization
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Uematsu Y
Kiefer H
Schulze R
Fischer-Lindahl K
Steinmetz M
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-09-00
Pages
2123-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167091
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source PMC Full Text View journal details
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