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PMID: 19509053 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Dynamic adhesions and MARCKS in melanoma cells.

Journal of cell science ·Vol. 122 ·No. Pt 13 ·2009-07-01 ·Pages 2300-10

Estrada-Bernal A, Gatlin JC, Sunpaweravong S, Pfenninger KH

Abstract

Cell motility necessitates the rapid formation and disassembly of cell adhesions. We have studied adhesions in a highly motile melanoma cell line using various biochemical approaches and microscopic techniques to image close adhesions. We report that WM-1617 melanoma cells contain at least two types of close adhesion: classic focal adhesions and more extensive, irregularly shaped adhesions that tend to occur along lamellipodial edges. In contrast to focal adhesions, these latter adhesions are highly dynamic and can be disassembled rapidly via protein kinase C (PKC) activation (e.g. by eicosanoid) and MARCKS phosphorylation. MARCKS overexpression, however, greatly increases the area of close adhesions and renders them largely refractory to PKC stimulation. This indicates that nonphosphorylated MARCKS is an adhesion stabilizer. Unlike focal adhesions, the dynamic adhesions contain alpha3 integrin and MARCKS, but they do not contain the focal adhesion marker vinculin. Overall, these results begin to define the molecular and functional properties of dynamic close adhesions involved in cell motility.

MeSH Terms
Animals Cell Adhesion Cell Line, Tumor Cell Movement Humans Integrin alpha3/metabolism Intercellular Junctions/physiology Intracellular Signaling Peptides and Proteins/genetics,metabolism Isoenzymes/genetics,metabolism Melanoma/metabolism,pathology Melanoma, Experimental/metabolism,pathology Membrane Proteins/genetics,metabolism Mice Myristoylated Alanine-Rich C Kinase Substrate Paxillin/metabolism Protein Kinase C/genetics,metabolism Rats Recombinant Fusion Proteins/genetics,metabolism Tetradecanoylphorbol Acetate/metabolism Vinculin/metabolism
Chemicals
Integrin alpha3 Intracellular Signaling Peptides and Proteins Isoenzymes MARCKS protein, human Marcks protein, mouse Marcks protein, rat Membrane Proteins Paxillin Recombinant Fusion Proteins Myristoylated Alanine-Rich C Kinase Substrate Vinculin Protein Kinase C Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Estrada-Bernal Adriana
Department of Pediatrics, University of Colorado School of Medicine, University of Colorado Cancer Center, and Colorado Intellectual and Developmental Disabilities Research Center, Aurora, CO 80045, USA.
Gatlin Jesse C
Sunpaweravong Somkiat
Pfenninger Karl H
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Article Info
Journal
Journal of cell science
Abbr.
J Cell Sci
ISSN
0021-9533
DOI
10.1242/jcs.047860
Published
2009-07-01
Epub
2009-00-09
Pages
2300-10
Language
English
Region
England
NLM ID
0052457
PMCID
PMC2723148
Subset
IM
Grants
NINDS NIH HHS · R01 NS41029 · United States
External Links
PubMed source DOI: 10.1242/jcs.047860 PMC Full Text View journal details
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