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PMID: 1986237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo pre-tRNA processing in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 425-39

O'Connor JP, Peebles CL

Abstract

We have surveyed intron-containing RNAs of the yeast Saccharomyces cerevisiae by filter hybridization with pre-tRNA intron-specific oligonucleotide probes. We have classified various RNAs as pre-tRNAs, splicing intermediates, or excised intron products according to apparent size and structure. Linear, excised intron products were detected, and one example was isolated and sequenced directly. Additional probes designed to detect other precursor sequences were used to verify the identification of several intermediates. Pre-tRNA species with both 5' leader and 3' extension, with 3' extension only, and with mature ends were distinguished. From these results, we conclude that the processing reactions used to remove the 5' leader and 3' extension from the transcript are ordered 5' end trimming before 3' end trimming. Splicing intermediates containing the 5' exon plus the intron were detected. The splice site cleavage reactions are probably ordered 3' splice site cleavage before 5' splice site cleavage. Surprisingly, we also detected a splicing intermediate with the 5' leader and a spliced product with both 5' leader and 3' extension. Evidently, splicing and end trimming are not ordered relative to each other, splicing occurring either before or after end trimming.

MeSH Terms
Base Sequence Introns Molecular Sequence Data Molecular Weight Nucleic Acid Precursors/metabolism RNA Processing, Post-Transcriptional RNA Splicing RNA, Fungal/metabolism RNA, Transfer/metabolism Saccharomyces cerevisiae/metabolism
Chemicals
Nucleic Acid Precursors RNA, Fungal RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Connor J P
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Peebles C L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.425-439.1991
Published
1991-01-00
Pages
425-39
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359644
Subset
IM
Grants
NCRR NIH HHS · 2S07 RR07084 · United States
NIGMS NIH HHS · GM32511 · United States
NIGMS NIH HHS · GM37166 · United States
Databases
GENBANK
M63607, M64827, S43602, S43604, S43609, S43613, S43614, S43616, S43620, S43862
External Links
PubMed source DOI: 10.1128/mcb.11.1.425-439.1991 PMC Full Text View journal details
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