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PMID: 2014164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoters with the octamer DNA motif (ATGCAAAT) can be ubiquitous or cell type-specific depending on binding affinity of the octamer site and Oct-factor concentration.

Nucleic acids research ·Vol. 19 ·No. 2 ·1991-01-25 ·Pages 237-42

Kemler I, Bucher E, Seipel K, Müller-Immerglück MM, Schaffner W

Abstract

Immunoglobulin (Ig) gene promoters contain the octamer sequence motif ATGCAAAT which is recognized by cellular transcription factors (Oct factors). Besides the ubiquitous Oct-1 factor, there is also a group of related factors (Oct-2 factors) encoded by a separate gene. The Oct-2 gene is regulated in a cell-type specific manner, and the protein is present in large amounts in B lymphocytes. We have previously shown that simple composite promoters of an octamer/TATA box type are poorly active in non-B cells but are strongly responsive to ectopic expression of Oct-2A factor, a major representative of the lymphocyte Oct-2 factors. In the present study we have tested the activity of a number of composite promoters and natural Ig promoters, and their response to Oct-1 and Oct-2 factors. Unexpectedly, we find that octamer/TATA promoters with a high affinity octamer site direct ubiquitous expression. By contrast, promoter constructions that behave in a B cell-specific manner tend to have a weak octamer binding site. These promoters are responsive to ectopic expression of additional Oct-factor, irrespective of whether it is Oct-1 or Oct-2. Using natural Ig promoters rather than composite promoters, we find that an IgH promoter is well transcribed in non-B cells via the ubiquitous Oct-1 factor, while Ig kappa and Ig lambda light chain promoters require additional Oct factor for maximal expression. It seems therefore likely that during B cell differentiation, Ig heavy chain promoters can be activated by Oct-1, before the appearance of Oct-2 factors. Oct-2 factors then would serve to boost the expression from Ig light chain promoters, which are known to be activated only after successful heavy chain gene rearrangement.

MeSH Terms
Autoradiography Base Sequence DNA/genetics DNA-Binding Proteins/genetics Gene Expression Regulation HeLa Cells Host Cell Factor C1 Immunoglobulins/genetics Molecular Sequence Data Octamer Transcription Factor-1 Plasmids Promoter Regions, Genetic TATA Box Transcription Factors/genetics Transcription, Genetic Transfection
Chemicals
DNA-Binding Proteins Host Cell Factor C1 Immunoglobulins Octamer Transcription Factor-1 Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kemler I
Institut für Molekularbiologie II, Universität Zürich, Switzerland.
Bucher E
Seipel K
Müller-Immerglück M M
Schaffner W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.2.237
Published
1991-01-25
Pages
237-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333585
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.2.237 PMC Full Text View journal details
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