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PMID: 20969679 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Reducing PEX13 expression ameliorates physiological defects of late-acting peroxin mutants.

Traffic (Copenhagen, Denmark) ·Vol. 12 ·No. 1 ·2011-01-00 ·Pages 121-34

Ratzel SE, Lingard MJ, Woodward AW, Bartel B

Abstract

Proteins are targeted to the peroxisome matrix via processes that are mechanistically distinct from those used by other organelles. Protein entry into peroxisomes requires peroxin (PEX) proteins, including early-acting receptor (e.g. PEX5) and docking peroxins (e.g. PEX13 and PEX14) and late-acting PEX5-recycling peroxins (e.g. PEX4 and PEX6). We examined genetic interactions among Arabidopsis peroxin mutants and found that the weak pex13-1 allele had deleterious effects when combined with pex5-1 and pex14-2, which are defective in early-acting peroxins, as shown by reduced matrix protein import and enhanced physiological defects. In contrast, combining pex13-1 with pex4-1 or pex6-1, which are defective in late-acting peroxins, unexpectedly ameliorated mutant growth defects. Matrix protein import remained impaired in pex4-1 pex13-1 and pex6-1 pex13-1, suggesting that the partial suppression of pex4-1 and pex6-1 physiological defects by a weak pex13 allele may result from restoring the balance between import and export of PEX5 or other proteins that are retrotranslocated from the peroxisome with the assistance of PEX4 and PEX6. Our results suggest that symptoms caused by pex mutants defective in late-acting peroxins may result not only from defects in matrix protein import but also from inefficient removal of PEX5 from the peroxisomal membrane following cargo delivery.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Cell Cycle Proteins/metabolism Down-Regulation Gene Expression Regulation, Plant Models, Biological Mutation Peroxins Peroxisomes/metabolism RNA/metabolism
Chemicals
Arabidopsis Proteins Cell Cycle Proteins PEX13 protein, Arabidopsis Peroxins RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ratzel Sarah E
Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA.
Lingard Matthew J
Woodward Andrew W
Bartel Bonnie
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Article Info
Journal
Traffic (Copenhagen, Denmark)
Abbr.
Traffic
ISSN
1600-0854
DOI
10.1111/j.1600-0854.2010.01136.x
Published
2011-01-00
Epub
2010-00-24
Pages
121-34
Language
English
Region
England
NLM ID
100939340
PMCID
PMC3005767
Subset
IM
Grants
NIGMS NIH HHS · R01 GM079177 · United States
NIGMS NIH HHS · R01GM079177 · United States
NIGMS NIH HHS · R01 GM079177-02 · United States
NIGMS NIH HHS · R01 GM079177-03 · United States
NIGMS NIH HHS · R01 GM079177-01A2 · United States
External Links
PubMed source DOI: 10.1111/j.1600-0854.2010.01136.x PMC Full Text View journal details
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