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PMID: 2123291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Activation of octamer-containing promoters by either octamer-binding transcription factor 1 (OTF-1) or OTF-2 and requirement of an additional B-cell-specific component for optimal transcription of immunoglobulin promoters.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6204-15

Pierani A, Heguy A, Fujii H, Roeder RG

Abstract

Several distinct octamer-binding transcription factors (OTFs) interact with the sequence ATTTGCAT (the octamer motif), which acts as a transcription regulatory element for a variety of differentially controlled genes. The ubiquitous OTF-1 plays a role in expression of the cell cycle-regulated histone H2b gene as well as several other genes, while the tissue-specific OTF-2 has been implicated in the tissue-specific expression of immunoglobulin genes. In an attempt to understand the apparent transcriptional selectivity of these factors, we have investigated the physical and functional characteristics of OTF-1 purified from HeLa cells and both OTF-1 and OTF-2 purified from B cells. High-resolution footprinting and mobility shift-competition assays indicated that these factors were virtually indistinguishable in binding affinities and DNA-protein contacts on either the H2b or an immunoglobulin light-chain (kappa) promoter. In addition, each of the purified factors showed an equivalent intrinsic capacity to activate transcription from either immunoglobulin promoters (kappa and heavy chain) or the H2b promoter in OTF-depleted HeLa and B-cell extracts. However, with OTF-depleted HeLa extracts, neither factor could restore immunoglobulin gene transcription to the relatively high level observed in unfractionated B-cell extracts. Restoration of full immunoglobulin gene activity appears to require an additional B-cell regulatory component which interacts with the OTFs. The additional B-cell factor could act either by facilitating interaction of OTF activation domains with components of the general transcriptional machinery or by contributing a novel activation domain.

MeSH Terms
Base Sequence Cloning, Molecular DNA-Binding Proteins/metabolism Deoxyribonuclease I Gene Expression Regulation Genes, Immunoglobulin HeLa Cells/immunology,metabolism Histones/genetics Host Cell Factor C1 Humans Immunoglobulin Light Chains/genetics Kinetics Molecular Sequence Data Nucleotide Mapping Octamer Transcription Factor-1 Octamer Transcription Factor-2 Oligonucleotide Probes Plasmids Promoter Regions, Genetic Restriction Mapping Transcription Factors/metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins HCFC1 protein, human Histones Host Cell Factor C1 Immunoglobulin Light Chains Octamer Transcription Factor-1 Octamer Transcription Factor-2 Oligonucleotide Probes POU2F1 protein, human POU2F2 protein, human Transcription Factors Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pierani A
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Heguy A
Fujii H
Roeder R G
References (63)
63 references, click to expand
  1. OVEC, a versatile system to study transcription in mammalian cells and cell-free extracts.
    Nucleic Acids Res. 1987 Sep 11;15(17):6787-98 PMID: 3658668
  2. The DNA binding domain (POU domain) of transcription factor oct-1 suffices for stimulation of DNA replication.
    EMBO J. 1990 Jun;9(6):1883-8 PMID: 2347308
  3. Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.
    Cell. 1987 Dec 4;51(5):783-93 PMID: 3119226
  4. Herpes simplex virus regulatory elements and the immunoglobulin octamer domain bind a common factor and are both targets for virion transactivation.
    Cell. 1988 Feb 12;52(3):435-45 PMID: 2830987
  5. Cell-cycle regulation of a human histone H2b gene is mediated by the H2b subtype-specific consensus element.
    Genes Dev. 1988 Jan;2(1):32-9 PMID: 3128460
  6. Purification of a nuclear trans-acting factor involved in the regulated transcription of a human immunoglobulin heavy chain gene.
    Cell. 1988 Jun 3;53(5):723-30 PMID: 2836066
  7. A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6347-51 PMID: 2842768
  8. Common mechanisms of promoter recognition by RNA polymerases II and III.
    Trends Genet. 1989 Apr;5(4):122-6 PMID: 2658227
  9. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
    Science. 1989 Jul 28;245(4916):371-8 PMID: 2667136
  10. Eukaryotic transcriptional regulatory proteins.
    Annu Rev Biochem. 1989;58:799-839 PMID: 2673023
  11. The Oct-1 homoeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16.
    Nature. 1989 Oct 19;341(6243):624-30 PMID: 2571937
  12. A family of octamer-specific proteins present during mouse embryogenesis: evidence for germline-specific expression of an Oct factor.
    EMBO J. 1989 Sep;8(9):2543-50 PMID: 2573523
  13. Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III.
    Science. 1988 Sep 2;241(4870):1210-3 PMID: 3413485
  14. A human protein specific for the immunoglobulin octamer DNA motif contains a functional homeobox domain.
    Cell. 1988 Oct 7;55(1):135-44 PMID: 2901913
  15. Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly.
    Cell. 1988 Oct 21;55(2):211-9 PMID: 2844412
  16. How eukaryotic transcriptional activators work.
    Nature. 1988 Oct 20;335(6192):683-9 PMID: 3050531
  17. A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.
    Nature. 1988 Dec 8;336(6199):544-51 PMID: 2904653
  18. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
    Nature. 1988 Dec 8;336(6199):551-7 PMID: 2904654
  19. Drosophila homoeotic genes encode transcriptional activators similar to mammalian OTF-2.
    Nature. 1988 Dec 8;336(6199):598-601 PMID: 2904655
  20. The POU domain is a bipartite DNA-binding structure.
    Nature. 1988 Dec 8;336(6199):601-4 PMID: 2904656
  21. Octamer-binding proteins from B or HeLa cells stimulate transcription of the immunoglobulin heavy-chain promoter in vitro.
    Genes Dev. 1988 Oct;2(10):1227-37 PMID: 3264542
  22. The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products.
    Genes Dev. 1988 Dec;2(12A):1513-6 PMID: 3215510
  23. The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains.
    Genes Dev. 1988 Dec;2(12A):1570-81 PMID: 3265124
  24. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  25. Functional cooperativity between protein molecules bound at two distinct sequence elements of the immunoglobulin heavy-chain promoter.
    Nature. 1989 Feb 9;337(6207):573-6 PMID: 2492641
  26. How do different transcription factors binding the same DNA sequence sort out their jobs?
    Trends Genet. 1989 Feb;5(2):37-9 PMID: 2646793
  27. Separation of requirements for protein-DNA complex assembly from those for functional activity in the herpes simplex virus regulatory protein Vmw65.
    J Virol. 1989 Apr;63(4):1641-50 PMID: 2538647
  28. Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography.
    Proc Natl Acad Sci U S A. 1989 Mar;86(6):1781-5 PMID: 2648381
  29. Identification of a novel lymphoid specific octamer binding protein (OTF-2B) by proteolytic clipping bandshift assay (PCBA).
    EMBO J. 1988 Dec 20;7(13):4221-9 PMID: 3072196
  30. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  31. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
    J Biol Chem. 1980 Dec 25;255(24):11992-6 PMID: 7440580
  32. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  33. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  34. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  35. Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequence.
    Cell. 1985 Jun;41(2):479-87 PMID: 3921262
  36. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements.
    Cell. 1985 Jul;41(3):885-97 PMID: 3924411
  37. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  38. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication.
    Nature. 1986 Aug 14-20;322(6080):656-9 PMID: 3748145
  39. Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6382-6 PMID: 3462701
  40. The mouse immunoglobulin heavy-chain enhancer: effect on transcription in vitro and binding of proteins present in HeLa and lymphoid B cell extracts.
    EMBO J. 1986 Aug;5(8):1791-7 PMID: 3019664
  41. Interaction of cell-type-specific nuclear proteins with immunoglobulin VH promoter region sequences.
    Nature. 1986 Oct 9-15;323(6088):548-51 PMID: 3093895
  42. A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.
    Nature. 1986 Oct 16-22;323(6089):640-3 PMID: 3095662
  43. Cell-type-specific transcription of an immunoglobulin kappa light chain gene in vitro.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8511-5 PMID: 3095838
  44. Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene.
    Mol Cell Biol. 1986 Oct;6(10):3329-40 PMID: 3025588
  45. Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication.
    Mol Cell Biol. 1987 Feb;7(2):875-86 PMID: 3821731
  46. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  47. Cell type-specificity elements of the immunoglobulin heavy chain gene enhancer.
    EMBO J. 1987 May;6(5):1323-30 PMID: 3038516
  48. An octamer oligonucleotide upstream of a TATA motif is sufficient for lymphoid-specific promoter activity.
    Nature. 1987 Sep 10-16;329(6135):174-8 PMID: 3627254
  49. The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.
    Cell. 1987 Oct 9;51(1):81-7 PMID: 3652210
  50. Herpes simplex virus Vmw65-octamer binding protein interaction: a paradigm for combinatorial control of transcription.
    Virology. 1989 Dec;173(2):363-7 PMID: 2556838
  51. Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
    Cell. 1989 Dec 22;59(6):1071-80 PMID: 2532066
  52. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.
    Cell. 1990 Feb 9;60(3):375-86 PMID: 2302733
  53. Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro.
    Genes Dev. 1989 Dec;3(12A):1982-90 PMID: 2620829
  54. Octamer-binding proteins in diverse hemopoietic cells.
    Mol Cell Biol. 1990 Mar;10(3):1293-6 PMID: 2106071
  55. The ubiquitous octamer-binding protein(s) is sufficient for transcription of immunoglobulin genes.
    Mol Cell Biol. 1990 Mar;10(3):982-90 PMID: 2304473
  56. Immunoglobulin gene transcription: molecular mechanisms.
    Trends Genet. 1989 Dec;5(12):395-9 PMID: 2696182
  57. Octamer transcription factors and the cell type-specificity of immunoglobulin gene expression.
    FASEB J. 1990 Mar;4(5):1444-9 PMID: 2407588
  58. An upstream transcription factor, USF (MLTF), facilitates the formation of preinitiation complexes during in vitro chromatin assembly.
    EMBO J. 1990 Apr;9(4):1299-308 PMID: 2323340
  59. Identification of a novel factor that interacts with an immunoglobulin heavy-chain promoter and stimulates transcription in conjunction with the lymphoid cell-specific factor OTF2.
    Mol Cell Biol. 1990 May;10(5):2145-53 PMID: 2109187
  60. The cell type-specific octamer transcription factor OTF-2 has two domains required for the activation of transcription.
    EMBO J. 1990 May;9(5):1635-43 PMID: 2328729
  61. Complex regulation of the immunoglobulin mu heavy-chain gene enhancer: microB, a new determinant of enhancer function.
    Mol Cell Biol. 1990 Jun;10(6):3145-54 PMID: 2111446
  62. Involvement of a second lymphoid-specific enhancer element in the regulation of immunoglobulin heavy-chain gene expression.
    Mol Cell Biol. 1990 Jun;10(6):3155-62 PMID: 2111447
  63. Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.
    Cell. 1987 Dec 4;51(5):773-81 PMID: 3677172
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6204-6215.1990
Published
1990-12-00
Pages
6204-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362895
Subset
IM
Grants
NIAID NIH HHS · AI27397 · United States
NCI NIH HHS · CA42567 · United States
NCRR NIH HHS · RR05869 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.12.6204-6215.1990 PMC Full Text View journal details
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