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PMID: 2155407 Published · ppublish English Comparative Study Journal Article

Sequence dependent modulating effect of camptothecin on the DNA-cleaving activity of the calf thymus type I topoisomerase.

Nucleic acids research ·Vol. 18 ·No. 3 ·1990-02-11 ·Pages 637-45

Gromova II, Buchman VL, Abagyan RA, Ulyanov AV, Bronstein IB

Abstract

High-resolution mapping of topol cleavages in the regions of human DNA including the oncogene c-Ha-ras and p53, has revealed three kinds of topol cleavage sites: cleavage sites not affected by camptothecin; cleavage sites reinforced only in the presence of camptothecin, and cleavage sites which weaken in the presence of camptothecin. Statistical analysis of sequences revealed certain nucleotide or dinucleotide preferences for three groups studied. The preferences in camptothecin-reduced sites predominate upstream from the cleavage point, whereas in camptothecin-induced sites the situation is reversed. The influence of camptothecin on cleavage sites induced by two molecular forms of topol has been also studied.

MeSH Terms
Animals Base Sequence Camptothecin/pharmacology Cattle DNA/metabolism DNA Topoisomerases, Type I/metabolism Humans Molecular Sequence Data Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins p21(ras) Restriction Mapping Substrate Specificity Thymus Gland/enzymology
Chemicals
Proto-Oncogene Proteins DNA HRAS protein, human Proto-Oncogene Proteins p21(ras) DNA Topoisomerases, Type I Camptothecin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gromova I I
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Buchman V L
Abagyan R A
Ulyanov A V
Bronstein I B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.3.637
Published
1990-02-11
Pages
637-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333473
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.3.637 PMC Full Text View journal details
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