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PMID: 2157062 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Epstein-Barr virus BRLF1 immediate-early gene product transactivates the human immunodeficiency virus type 1 long terminal repeat by a mechanism which is enhancer independent.

Journal of virology ·Vol. 64 ·No. 4 ·1990-04-00 ·Pages 1817-20

Quinlivan EB, Holley-Guthrie E, Mar EC, Smith MS, Kenney S

Abstract

The Epstein-Barr virus (EBV) immediate-early gene product, BRLF1, transactivates the human immunodeficiency virus type 1 (HIV-1) long terminal repeat. BRLF1-induced transactivation of HIV-1 promoter constructs is accompanied by an increase in plasmid mRNA and is reporter gene independent. Previously, BRLF1 transactivation of EBV promoters has been mapped to regions which function as enhancer elements. Deletional analysis demonstrates that BRLF1 transactivation of the HIV-1 promoter does not require the HIV-1 enhancer. Thus, the EBV BRLF1 gene product may transactivate by at least two different mechanisms, one mechanism involving certain enhancer elements and another mechanism which is enhancer independent.

MeSH Terms
Antigens, Viral/physiology Cell Line Enhancer Elements, Genetic HIV-1/genetics Herpesvirus 4, Human/genetics Humans Mutation Promoter Regions, Genetic RNA, Messenger/genetics RNA, Viral/genetics Repetitive Sequences, Nucleic Acid Trans-Activators/physiology
Chemicals
Antigens, Viral Epstein-Barr virus early antigen RNA, Messenger RNA, Viral Trans-Activators
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Quinlivan E B
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27514.
Holley-Guthrie E
Mar E C
Smith M S
Kenney S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.4.1817-1820.1990
Published
1990-04-00
Pages
1817-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249321
Subset
IM
Grants
NIAID NIH HHS · 1-UO1-AI256868-0 · United States
NCI NIH HHS · CA 01229-03 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.4.1817-1820.1990 PMC Full Text View journal details
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