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PMID: 2167436 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evi-2, a common integration site involved in murine myeloid leukemogenesis.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4658-66

Buchberg AM, Bedigian HG, Jenkins NA, Copeland NG

Abstract

BXH-2 mice have the highest incidence of spontaneous retrovirally induced myeloid leukemia of any known inbred strain and, as such, represent a valuable model system for identifying cellular proto-oncogenes involved in myeloid disease. Chronic murine leukemia viruses often induce disease by insertional activation or mutation of cellular proto-oncogenes. These loci are identified as common viral integration sites in tumor DNAs. Here we report on the characterization of a novel common viral integration site in BXH-2 myeloid leukemias, designated Evi-2. Within the cluster of viral integration sites that define Evi-2, we identified a gene that has the potential for encoding a novel protein of 223 amino acids. This putative proto-oncogene possesses all of the structural features of a transmembrane protein. Within the transmembrane domain is a "leucine zipper," suggesting that Evi-2 is involved in either homopolymer or heteropolymer formation, which may play an important role in the normal functioning of Evi-2. Interestingly, the human homolog of Evi-2 has recently been shown to be tightly linked to the von Recklinghausen neurofibromatosis locus, suggesting a role for Evi-2 in human disease as well.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Probes DNA, Viral/genetics,isolation & purification Gene Expression Regulation, Viral Gene Library Information Systems Leukemia Virus, Murine/genetics,isolation & purification Leukemia, Experimental/genetics,microbiology Membrane Proteins/genetics Mice Mice, Inbred Strains Molecular Sequence Data Protein Conformation Proto-Oncogene Mas Proto-Oncogenes Recombination, Genetic Restriction Mapping Software Transcription, Genetic Viral Proteins/genetics
Chemicals
DNA Probes DNA, Viral Evi2a protein, mouse MAS1 protein, human Membrane Proteins Proto-Oncogene Mas Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buchberg A M
Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702.
Bedigian H G
Jenkins N A
Copeland N G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4658-4666.1990
Published
1990-09-00
Pages
4658-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361055
Subset
IM
Grants
NCI NIH HHS · CA31101 · United States
NCI NIH HHS · N01-CO-74101 · United States
Databases
GENBANK
M34896, M34897
External Links
PubMed source DOI: 10.1128/mcb.10.9.4658-4666.1990 PMC Full Text View journal details
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