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PMID: 22355166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles.

RNA (New York, N.Y.) ·Vol. 18 ·No. 4 ·2012-04-00 ·Pages 738-51

Miyagawa R, Tano K, Mizuno R, Nakamura Y, Ijiri K, Rakwal R, Shibato J, Masuo Y, Mayeda A, Hirose T, Akimitsu N

Abstract

MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote the localization of MALAT-1 to nuclear speckles and also provide evidence that MALAT-1 is involved in the regulation of gene expression. Heterokaryon assays revealed that MALAT-1 does not shuttle between the nucleus and cytoplasm. RNAi-mediated repression of the nuclear speckle proteins, RNPS1, SRm160, or IBP160, which are well-known mRNA processing factors, resulted in the diffusion of MALAT-1 to the nucleoplasm. We demonstrated that MALAT-1 contains two distinct elements directing transcripts to nuclear speckles, which were also capable of binding to RNPS1 in vitro. Depletion of MALAT-1 represses the expression of several genes. Taken together, our results suggest that RNPS1, SRm160, and IBP160 contribute to the localization of MALAT-1 to nuclear speckles, where MALAT-1 could be involved in regulating gene expression.

MeSH Terms
Base Sequence Cell Nucleus/metabolism DNA Primers Down-Regulation HeLa Cells Humans RNA Interference RNA, Long Noncoding RNA, Untranslated/genetics,metabolism
Chemicals
DNA Primers MALAT1 long non-coding RNA, human RNA, Long Noncoding RNA, Untranslated
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Miyagawa Ryu
Radioisotope Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
Tano Keiko
Mizuno Rie
Nakamura Yo
Ijiri Kenichi
Rakwal Randeep
Shibato Junko
Masuo Yoshinori
Mayeda Akila
Hirose Tetsuro
Akimitsu Nobuyoshi
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1469-9001
DOI
10.1261/rna.028639.111
Published
2012-04-00
Epub
2012-00-21
Pages
738-51
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC3312561
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.028639.111 PMC Full Text View journal details
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