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PMID: 2285280 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of chromosomal antibiotic resistance genes in Streptococcus anginosus ("S. milleri").

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 9 ·1990-09-00 ·Pages 1685-90

Clermont D, Horaud T

Abstract

Determinants encoding resistance to antibiotics, except penicillin, of 5 of 21 Streptococcus anginosus clinical isolates that we examined transferred by conjugation into Enterococcus faecalis and into 1 to 5 streptococcal recipients at frequencies of 10(-6) to 10(-8) transconjugants per donor. No R plasmids were detected in any wild-type strain. DNA homology was detected between chromosomal fragments and the following genes: aadE in 7 strains that were highly resistant to streptomycin, aph-3 in 8 strains that were highly resistant to kanamycin, ermB in 11 of 15 strains that were resistant to erythromycin, and tetM in 14 and tetO in 3 of the 18 strains that were resistant to tetracycline-minocycline. A Tn916-like structure was found in 10 of the 14 strains that carried the tetM gene.

Related Genes
Tn916 aadE aph-3 ermB tetM tetO
MeSH Terms
Chromosomes/physiology Conjugation, Genetic/physiology DNA Probes DNA, Bacterial/genetics Drug Resistance, Microbial/genetics Erythromycin/pharmacology Nucleic Acid Hybridization Plasmids/physiology Streptococcus/genetics Tetracycline Resistance/genetics
Chemicals
DNA Probes DNA, Bacterial Erythromycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clermont D
Faculté Odontologique, Université Paris VII, France.
Horaud T
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37 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.9.1685
Published
1990-09-00
Pages
1685-90
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171905
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.34.9.1685 PMC Full Text View journal details
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