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PMID: 2153659 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Presence of chromosomal elements resembling the composite structure Tn3701 in streptococci.

Journal of bacteriology ·Vol. 172 ·No. 2 ·1990-02-00 ·Pages 727-34

Le Bouguénec C, de Cespédès G, Horaud T

Abstract

Tn3701, carried by Streptococcus pyogenes A454, is the first chromosomal composite element to be described; it contains in its central region Tn3703, a transposon similar to Tn916. A comparison by DNA-DNA hybridization of Tn3701 with omega(cat-tet) and Tn3951, carried by Streptococcus pneumoniae BM6001 and by Streptococcus agalactiae B109, respectively, revealed that the two latter structures are also Tn3701-like composite elements. The DNAs of 27 other antibiotic-resistant group A, B, C, and G streptococci and of S. pneumoniae BM4200 showed sequence homologies to Tn3701 (14 strains, including BM4200), to the regions of Tn3701 outside of Tn3703 (5 strains), and to Tn916 alone (8 strains). The DNAs of five strains did not detectably hybridize with any probe. The tetM gene was identified in most chromosomal genetic elements coding for tetracycline-minocycline resistance. Since Tn3701-like elements are widely disseminated among antibiotic-resistant streptococci (47% of the 34 strains studied), we propose that Tn3701 be considered the prototype of chromosomal composite elements.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements Drug Resistance, Microbial/genetics Genes, Bacterial Genetic Markers Nucleic Acid Hybridization Sequence Homology, Nucleic Acid Streptococcus/genetics Streptococcus agalactiae/genetics Streptococcus pneumoniae/genetics Streptococcus pyogenes/genetics
Chemicals
DNA Transposable Elements Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Le Bouguénec C
Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur, Paris, France.
de Cespédès G
Horaud T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.2.727-734.1990
Published
1990-02-00
Pages
727-34
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208499
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.2.727-734.1990 PMC Full Text View journal details
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