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PMID: 23593021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential association of the conserved SUMO ligase Zip3 with meiotic double-strand break sites reveals regional variations in the outcome of meiotic recombination.

PLoS genetics ·Vol. 9 ·No. 4 ·2013-04-00 ·Pages e1003416

Serrentino ME, Chaplais E, Sommermeyer V, Borde V

Abstract

During the first meiotic prophase, programmed DNA double-strand breaks (DSBs) are distributed non randomly at hotspots along chromosomes, to initiate recombination. In all organisms, more DSBs are formed than crossovers (CO), the repair product that creates a physical link between homologs and allows their correct segregation. It is not known whether all DSB hotspots are also CO hotspots or if the CO/DSB ratio varies with the chromosomal location. Here, we investigated the variations in the CO/DSB ratio by mapping genome-wide the binding sites of the Zip3 protein during budding yeast meiosis. We show that Zip3 associates with DSB sites that are engaged in repair by CO, and Zip3 enrichment at DSBs reflects the DSB tendency to be repaired by CO. Moreover, the relative amount of Zip3 per DSB varies with the chromosomal location, and specific chromosomal features are associated with high or low Zip3 per DSB. This work shows that DSB hotspots are not necessarily CO hotspots and suggests that different categories of DSB sites may fulfill different functions.

MeSH Terms
Chromosomes, Fungal/genetics Crossing Over, Genetic DNA Breaks, Double-Stranded DNA Repair/genetics Homologous Recombination Meiosis/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics,metabolism Ubiquitin-Protein Ligases/genetics,metabolism
Chemicals
Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases Zip3 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Serrentino Maria-Elisabetta
Institut Curie, Centre de Recherche, Paris, France.
Chaplais Emmanuel
Sommermeyer Vérane
Borde Valérie
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1003416
Published
2013-04-00
Epub
2013-00-04
Pages
e1003416
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC3616913
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pgen.1003416 PMC Full Text View journal details
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