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PMID: 23856002 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Elongator subunit 3 positively regulates plant immunity through its histone acetyltransferase and radical S-adenosylmethionine domains.

BMC plant biology ·Vol. 13 ·2013-07-16 ·Pages 102

Defraia CT, Wang Y, Yao J, Mou Z

Abstract

Pathogen infection triggers a large-scale transcriptional reprogramming in plants, and the speed of this reprogramming affects the outcome of the infection. Our understanding of this process has significantly benefited from mutants that display either delayed or accelerated defense gene induction. In our previous work we demonstrated that the Arabidopsis Elongator complex subunit 2 (AtELP2) plays an important role in both basal immunity and effector-triggered immunity (ETI), and more recently showed that AtELP2 is involved in dynamic changes in histone acetylation and DNA methylation at several defense genes. However, the function of other Elongator subunits in plant immunity has not been characterized. In the same genetic screen used to identify Atelp2, we found another Elongator mutant, Atelp3-10, which mimics Atelp2 in that it exhibits a delay in defense gene induction following salicylic acid treatment or pathogen infection. Similarly to AtELP2, AtELP3 is required for basal immunity and ETI, but not for systemic acquired resistance (SAR). Furthermore, we demonstrate that both the histone acetyltransferase and radical S-adenosylmethionine domains of AtELP3 are essential for its function in plant immunity. Our results indicate that the entire Elongator complex is involved in basal immunity and ETI, but not in SAR, and support that Elongator may play a role in facilitating the transcriptional induction of defense genes through alterations to their chromatin.

MeSH Terms
Arabidopsis/enzymology,genetics,immunology,microbiology Arabidopsis Proteins/chemistry,genetics,metabolism Gene Expression Regulation, Plant Histone Acetyltransferases/chemistry,genetics,metabolism Plant Diseases/genetics,immunology,microbiology Plant Immunity Protein Structure, Tertiary Pseudomonas syringae/physiology S-Adenosylmethionine/immunology Salicylic Acid/immunology
Chemicals
Arabidopsis Proteins S-Adenosylmethionine ELO3 protein, Arabidopsis Histone Acetyltransferases Salicylic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Defraia Christopher T
Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, FL 32611, USA.
Wang Yongsheng
Yao Jiqiang
Mou Zhonglin
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Article Info
Journal
BMC plant biology
Abbr.
BMC Plant Biol
ISSN
1471-2229
DOI
10.1186/1471-2229-13-102
Published
2013-07-16
Epub
2013-00-16
Pages
102
Language
English
Region
England
NLM ID
100967807
PMCID
PMC3728140
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2229-13-102 PMC Full Text View journal details
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