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PMID: 2390785 Published · ppublish English Journal Article

Primary and secondary structure of the 25S rRNA from the dimorphic fungus Mucor racemosus.

Current genetics ·Vol. 17 ·No. 6 ·1990-06-00 ·Pages 499-506

Ji GE, Orlowski M

Abstract

A 9.76 Kb ribosomal DNA repeat unit from the nuclear genome of the dimorphic fungus Mucor racemosus (Zygomycetes) was identified using a hybridization probe from the yeast Saccharomyces cerevisiae (Ascomycetes). This material was cloned in Escherichia coli plasmids as four overlapping pieces and mapped with respect to cleavage sites for 12 restriction endonucleases. The nucleotide sequence of the complete 25S rRNA gene and flanking regions was determined. The 5' and 3' ends of the structural gene were identified by comparison with the published sequence for the S. cerevisiae gene. The Mucor gene was found to possess 3469 bp and have a GC content of 42.8%. It was compared with the homologous gene from several other eukaryotes and found to be most similar to that from Saccharomyces. A potential secondary structure of the putative RNA transcript consistent with the structures proposed for the E. coli and Saccharomyces molecules was constructed by computer modelling.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Fungal/analysis DNA, Ribosomal/analysis Genes, Fungal Molecular Sequence Data Mucor/genetics Nucleic Acid Conformation RNA, Fungal RNA, Ribosomal/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA, Fungal DNA, Ribosomal RNA, Fungal RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ji G E
Department of Microbiology, Louisiana State University, Baton Rouge 70803-1715.
Orlowski M
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00313078
Published
1990-06-00
Pages
499-506
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
M26190
External Links
PubMed source DOI: 10.1007/BF00313078 View journal details
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