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PMID: 2410855 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Long range RNA-RNA interactions in the 30 S ribosomal subunit of E. coli.

Nucleic acids research ·Vol. 13 ·No. 13 ·1985-07-11 ·Pages 4719-38

Spitnik-Elson P, Elson D, Avital S, Abramowitz R

Abstract

We have attempted to identify long-range interactions in the tertiary structure of RNA in the E. coli 30 S ribosome. Native subunits were cleaved with ribonuclease and separated into nucleoprotein fragments which were deproteinized and fractionated into multi-oligonucleotide complexes under conditions intended to preserve RNA-RNA interactions. The final products were denatured by urea and heat and their constituent oligonucleotides resolved and sequenced. Many complexes contained complementary sequences known to be bound together in the RNA secondary structure, attesting to the validity of the technique. Other co-migrating oligonucleotides, not joined in the secondary structure, contained mutually complementary sequences in locations that allow base-pairing interaction without disrupting pre-existing secondary structure. In seven instances the complementary relationship was found to have been preserved during phylogenetic diversification.

MeSH Terms
Base Sequence Electrophoresis Escherichia coli/genetics Microscopy, Electron Nucleic Acid Conformation RNA, Bacterial/analysis RNA, Ribosomal/analysis Ribosomal Proteins/analysis Ribosomes/analysis
Chemicals
RNA, Bacterial RNA, Ribosomal Ribosomal Proteins ribosomal protein S30
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Spitnik-Elson P
Elson D
Avital S
Abramowitz R
References (48)
48 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.13.4719
Published
1985-07-11
Pages
4719-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321822
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.13.4719 PMC Full Text View journal details
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