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PMID: 24177800 Published · ppublish English Journal Article

A maturase-like coding sequence downstream of the OXI2 gene of yeast mitochondrial DNA is interrupted by two GC clusters and a putative end-of-messenger signal.

Current genetics ·Vol. 8 ·No. 4 ·1984-05-00 ·Pages 307-17

Michel F

Abstract

By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major family of intronic open reading frames. The RF2 open reading frame is not continuous, however, for it is interrupted by two GC clusters, both of which ultimately result in a -1 frameshift. Comparison with RF1 reveals a third insertion. This is centered on an oligo nucleotide, AATAATATTCTTA, which is found (sometimes in a slightly modified form) downstream of ten proven or suspected protein coding genes, including RF1 and RF2, and is known to terminate the apocytochrome b messenger RNA. It is suggested from the known distribution of this putative "end-of-messenger" signal, that it could play an essential part in controlling the expression of several minor proteins, both intronic and non-intronic. The possibility of the RF2 sequence being functional in spite of its interruptions is also discussed.

Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Michel F
Centre de Génétique Moléculaire, Laboratoire Propre du Centre National de la Recherche Scientifique, Associé à l'Université Pierre et Marie Curie, 91190, Gif-sur-Yvette, France.
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00419729
Published
1984-05-00
Pages
307-17
Language
English
Region
United States
NLM ID
8004904
External Links
PubMed source DOI: 10.1007/BF00419729 View journal details
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