Home LiteratureArticle Details
PMID: 2437024 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence of HLA-A11: remarkable homology with HLA-A3 allows identification of residues involved in epitopes recognized by antibodies and T cells.

Immunogenetics ·Vol. 25 ·No. 4 ·1987-00-00 ·Pages 241-50

Cowan EP, Jelachich ML, Biddison WE, Coligan JE

Abstract

The human class I alleles HLA-A11 and HLA-A3 have a well-documented history of serological cross-reactivity. This cross-reactivity suggests that they are closely related, a suggestion which is supported by the fact that the HLA-A11 and HLA-A3 genes are distinguished from all other A-locus genes by a restriction fragment length polymorphism observed in Bam HI digests. To examine the extent of sequence homology between HLA-A11 and HLA-A3, we have cloned the HLA-A11 gene and sequenced the coding regions (exons). The results reveal that HLA-A11 and HLA-A3 display the highest degree of homology reported for any pair of serologically defined class I alleles. Only nine base differences resulting in six amino acid differences were observed in exons 2-8. One of the amino acid substitutions is in the alpha 1 domain and the other five are in the alpha 2 domain. comparison of this sequence with that of other human class I molecules implicates Gln62 as a critical residue involved in HLA-A11 - HLA-A3 serological cross-reactivity. In addition, the amino acid sequence allowed us to successfully predict cross-reactive recognition of HLA-A11 by cytotoxic T lymphocytes specific for a rare subtype of HLA-A3, HLA-A3.2. This result provides further support for the importance of the alpha 2 domain residues 152 and 156 in forming determinants on class I molecules that are recognized by cytotoxic T lymphocytes.

MeSH Terms
Alleles Amino Acid Sequence Antibodies/immunology Base Sequence Cloning, Molecular Cross Reactions DNA/genetics Epitopes/genetics,immunology Exons HLA Antigens/genetics,immunology HLA-A Antigens HLA-A11 Antigen HLA-A3 Antigen Humans T-Lymphocytes, Cytotoxic/immunology
Chemicals
Antibodies Epitopes HLA Antigens HLA-A Antigens HLA-A11 Antigen HLA-A3 Antigen DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cowan E P
Jelachich M L
Biddison W E
Coligan J E
References (54)
54 references, click to expand
  1. Serological expression after sequential double transfection with purified HLA-A11 gene of mouse fibroblasts carrying human beta-2 microglobulin.
    Immunogenetics. 1985;22(1):1-8 PMID: 3894226
  2. Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815.
    Eur J Immunol. 1985 Nov;15(11):1111-7 PMID: 2933264
  3. Comparison of multiple HLA-A alleles at the DNA level by using Southern blotting and HLA-A-specific probes.
    J Immunol. 1985 Dec;135(6):4229-34 PMID: 2415606
  4. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  5. Recombination within the HLA-D region. Correlation of molecular genotyping with functional data.
    J Exp Med. 1984 Jul 1;160(1):222-38 PMID: 6330268
  6. Selection of highly transfectable variant from mouse mastocytoma P815.
    Somat Cell Mol Genet. 1985 Sep;11(5):467-75 PMID: 3929396
  7. The complete primary structure of HLA-Bw58.
    J Biol Chem. 1985 Oct 5;260(22):11924-33 PMID: 2995352
  8. Cloning and complete sequence of an HLA-A2 gene: analysis of two HLA-A alleles at the nucleotide level.
    J Immunol. 1985 Apr;134(4):2727-33 PMID: 2982951
  9. Structural analysis of the functional sites of class I HLA antigens.
    Immunol Rev. 1985 Jul;85:149-68 PMID: 2412949
  10. One allogeneic cytolytic T lymphocyte clone distinguishes three different HLA-B27 subtypes: identification of amino acid residues influencing the specificity and avidity of recognition.
    J Immunol. 1985 Nov;135(5):3074-81 PMID: 2413111
  11. T cell subpopulations required for the human cytotoxic T lymphocyte response to influenza virus: evidence for T cell help.
    J Immunol. 1981 Aug;127(2):487-91 PMID: 6788843
  12. Structure of class I major histocompatibility antigens.
    Contemp Top Mol Immunol. 1983;9:1-63 PMID: 6191921
  13. Human cytotoxic T-lymphocyte recognition of an HLA-A3 gene product expressed on murine L cells: the only human gene product required on the target cells for lysis is the class I heavy chain.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4490-4 PMID: 3892536
  14. Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: implications for the evolution of HLA genes.
    EMBO J. 1984 Apr;3(4):887-94 PMID: 6609814
  15. Localization of allodeterminants on H-2Kb antigens determined with monoclonal antibodies and H-2 mutant mice.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4737-41 PMID: 6181513
  16. Structural analysis of an HLA-B7 antigen variant detected by cytotoxic T lymphocytes.
    J Immunol. 1984 Aug;133(2):816-21 PMID: 6429245
  17. Monoclonal antibodies for analysis of the HLA system.
    Immunol Rev. 1979;47:3-61 PMID: 95015
  18. Transfection of murine LMTK- cells with purified HLA class I genes. VII. Association of allele- and locus-specific serological reactivities with respectively the first and second domains of the HLA-B7 molecule.
    Immunogenetics. 1986;23(4):246-51 PMID: 2422117
  19. Human major histocompatibility complex class I antigens: residues 61-83 of the HLA-B7 heavy chain specify an alloreactive site.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):539-42 PMID: 3881768
  20. Comparative structural analysis of HLA-A2 antigens distinguishable by cytotoxic T lymphocytes: variants M7 and DR1.
    Biochemistry. 1982 Nov 23;21(24):6313-21 PMID: 6983890
  21. Primary structure of papain-solubilized human histocompatibility antigen HLA-B40 (-Bw60). An outline of alloantigenic determinants.
    Biochemistry. 1983 Aug 2;22(16):3961-9 PMID: 6193808
  22. The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.
    Immunogenetics. 1985;21(5):479-89 PMID: 2987115
  23. Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3).
    EMBO J. 1984 Apr;3(4):879-85 PMID: 6609813
  24. Recognition of HLA class I molecules by antisera directed to synthetic peptides corresponding to different regions of the HLA-B7 heavy chain.
    J Immunol. 1986 Mar 1;136(5):1738-44 PMID: 3081631
  25. Comparative structural analysis of HLA-A3 antigens distinguishable by cytotoxic T lymphocytes: variant E1.
    J Immunol. 1985 Jan;134(1):410-6 PMID: 3871112
  26. HLA-B27 antigenicity: antibodies against the chemically synthesized 63-84 peptide from HLA-B27.1 display alloantigenic specificity and discriminate among HLA-B27 subtypes.
    J Immunol. 1986 Aug 1;137(3):904-10 PMID: 2424988
  27. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  28. Molecular analysis of an HLA-A2 functional variant CLA defined by cytolytic T lymphocytes.
    J Immunol. 1986 Sep 1;137(5):1642-9 PMID: 3489037
  29. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  30. Class I genes and molecules: an update.
    Immunology. 1986 Jan;57(1):3-18 PMID: 2417940
  31. DNA sequence of the coding region of the HLA-B44 gene.
    Immunogenetics. 1986;23(6):396-400 PMID: 3459708
  32. Altered structure of HLA class I heavy chains associated with mouse beta-2 microglobulin.
    Immunogenetics. 1985;21(4):321-31 PMID: 2581890
  33. Primary structure of papain-solubilized human histocompatibility antigen HLA-B27.
    Biochemistry. 1985 Mar 26;24(7):1733-41 PMID: 2408663
  34. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  35. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  36. A monoclonal antibody against HLA-A11 and A24.
    Hum Immunol. 1986 Mar;15(3):316-9 PMID: 2420768
  37. Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens.
    Nature. 1982 Dec 23;300(5894):755-7 PMID: 6184620
  38. The self determinants recognized by human virus-immune T cells can be distinguished from the serologically defined HLA antigens.
    J Immunol. 1980 Feb;124(2):548-52 PMID: 6153199
  39. Serological cross-reactivity in the HL-A system.
    Tissue Antigens. 1974;4(2):146-56 PMID: 4857913
  40. Amino acid residues 56 to 69 of HLA-A2 specify an antigenic determinant shared by HLA-A2 and HLA-B17.
    J Immunol. 1986 Jul 1;137(1):217-22 PMID: 2423602
  41. Exon shuffling in vivo can generate novel HLA class I molecules.
    EMBO J. 1985 Nov;4(11):2849-54 PMID: 3877632
  42. Structure of crossreactive human histocompatibility antigens HLA-A28 and HLA-A2: possible implications for the generation of HLA polymorphism.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3813-7 PMID: 6179086
  43. Comparative structural analysis of HLA-A2 antigens distinguishable by cytotoxic T lymphocytes. II. Variant DK1: evidence for a discrete CTL recognition region.
    J Immunol. 1983 Apr;130(4):1856-62 PMID: 6601143
  44. Isolation of HLA locus-specific DNA probes from the 3'-untranslated region.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175-8 PMID: 6591185
  45. The monoclonal antibody CR11-351 discriminates HLA-A2 variants identified by T cells.
    Immunogenetics. 1983;18(1):23-35 PMID: 6190744
  46. Variability and conformation of HLA class I antigens: a predictive approach to the spatial arrangement of polymorphic regions.
    Biochemistry. 1984 Feb 28;23(5):823-31 PMID: 6585230
  47. Murine major histocompatibility complex class-I mutants: molecular analysis and structure-function implications.
    Annu Rev Immunol. 1986;4:471-502 PMID: 3518748
  48. Structural analysis of an HLA-B27 functional variant: identification of residues that contribute to the specificity of recognition by cytolytic T lymphocytes.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7394-8 PMID: 2414775
  49. Sequences of human repetitive DNA, non-alpha-globin genes, and major histocompatibility locus genes.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1079-86 PMID: 6574860
  50. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  51. Serological distinction of mutants B6.CH(zl) and B6.M505 from strain C57BL-6.
    J Exp Med. 1974 Oct 1;140(4):1127-32 PMID: 4139224
  52. Delineation of functional sites in HLA-B27 antigens. Molecular analysis of HLA-B27 variant Wewak I defined by cytolytic T lymphocytes.
    J Immunol. 1985 Nov;135(5):3323-32 PMID: 3930603
  53. Cross-reactions of HL-A antibodies. II. Continuous pH gradient elution.
    J Immunol. 1972 Oct;109(4):681-5 PMID: 4561470
  54. Molecular cloning and DNA sequence analysis of genes encoding cytotoxic T lymphocyte-defined HLA-A3 subtypes: the E1 subtype.
    J Immunol. 1985 Oct;135(4):2835-41 PMID: 2993417
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00404694
Published
1987-00-00
Pages
241-50
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
M16007, M16008, M16009, M16010
External Links
PubMed source DOI: 10.1007/BF00404694 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2