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PMID: 2476660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activity of the carbamyl phosphate synthetase I promoter in liver nuclear extracts is dependent on a cis-acting C/EBP recognition element.

Molecular and cellular biology ·Vol. 9 ·No. 7 ·1989-07-00 ·Pages 2928-33

Howell BW, Lagacé M, Shore GC

Abstract

We have identified an essential cis element in the proximal promoter region of the rat carbamyl phosphate synthetase I (CPSI) gene that is requisite for promoter activity in liver nuclear extracts. Excess synthetic oligonucleotides specifying this region abolished promoter-dependent in vitro transcription. We show that C/EBP, a nuclear factor enriched in liver but found as well in other tissues, such as gut, fat, and lung, interacts with an inverted repeat, GTTGCAAC, at the core of the essential cis element. In brain, a tissue that did not express CPSI or contain significant levels of C/EBP, a different factor was capable of binding at or near the C/EBP recognition element. Activity of the CPSI promoter in liver nuclear extracts was also dependent on sequences 5' to the C/EBP motif; presumably, factors binding to elements within this upstream region are instrumental in restricting CPSI gene expression to liver and intestinal mucosa.

MeSH Terms
Animals Base Sequence Binding, Competitive Carbamoyl-Phosphate Synthase (Ammonia)/genetics,metabolism Cell Nucleus/enzymology DNA/genetics,metabolism Deoxyribonuclease I In Vitro Techniques Liver/cytology,enzymology Methylation Molecular Sequence Data Oligonucleotides/chemical synthesis,metabolism Organ Specificity Plasmids Promoter Regions, Genetic RNA/biosynthesis,genetics Rats Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Transcription Factors/metabolism
Chemicals
Oligonucleotides Transcription Factors RNA DNA Deoxyribonuclease I Carbamoyl-Phosphate Synthase (Ammonia)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Howell B W
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Lagacé M
Shore G C
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.7.2928-2933.1989
Published
1989-07-00
Pages
2928-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362760
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.7.2928-2933.1989 PMC Full Text View journal details
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