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PMID: 2479820 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and expression of STK, a src-related gene in the simple metazoan Hydra attenuata.

Molecular and cellular biology ·Vol. 9 ·No. 10 ·1989-10-00 ·Pages 4141-51

Bosch TC, Unger TF, Fisher DA, Steele RE

Abstract

Both cDNA clones and a genomic DNA clone encoding a 509-amino-acid protein that is 64% similar to chicken pp60c-src were isolated from the simple metazoan Hydra attenuata. We have designated this gene STK, for src-type kinase. Features of the amino acid sequence of the protein encoded by the STK gene suggest that it is likely to be myristoylated and regulated by phosphorylation in a manner similar to that found for pp60c-src. The genomic sequence encoding the protein was found to be interrupted by at least two introns, one of which was located in a position identical to that of one of the introns in the chicken src gene. The STK gene was expressed during early development of H. attenuata and at high levels in the epithelial cells of adult polyps. Probing of Hydra proteins with an antibody to phosphotyrosine indicated that the major phosphotyrosine-containing protein in H. attenuata may be the STK protein itself. H. attenuata is the simplest organism from which a protein-tyrosine kinase gene has been isolated. The presence of such a gene in the evolutionarily ancient phylum Cnidaria suggests that protein-tyrosine kinase genes arose concomitantly with or shortly after the appearance of multicellular organisms.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Western Cloning, Molecular Gene Library Hydra/embryology,enzymology,genetics Molecular Sequence Data Phosphotyrosine Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins pp60(c-src) RNA/biosynthesis Restriction Mapping Sequence Homology, Nucleic Acid Tyrosine/analysis
Chemicals
Proto-Oncogene Proteins Phosphotyrosine Tyrosine RNA Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bosch T C
Department of Biological Chemistry, University of California, Irvine 92717.
Unger T F
Fisher D A
Steele R E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.10.4141-4151.1989
Published
1989-10-00
Pages
4141-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362492
Subset
IM
Grants
NICHD NIH HHS · HD-07029 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.10.4141-4151.1989 PMC Full Text View journal details
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