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PMID: 2494352 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence, structure, and phylogenetic relationship of the small subunit rRNA coding region of mitochondrial DNA from Podospora anserina.

Journal of molecular evolution ·Vol. 28 ·No. 3 ·1989-03-00 ·Pages 232-41

Cummings DJ, Domenico JM, Nelson J, Sogin ML

Abstract

DNA sequence analysis and the localization of the 5' and 3' termini by S1 mapping have shown that the mitochondrial (mt) small subunit rRNA coding region from Podospora anserina is 1980 bp in length. The analogous coding region for mt rRNA is 1962 bp in maize, 1686 bp in Saccharomyces cerevisiae, and 956 bp in mammals, whereas its counterpart in Escherichia coli is 1542 bp. The P. anserina mt 16S-like rRNA is 400 bases longer than that from E. coli, but can be folded into a similar secondary structure. The additional bases appear to be clustered at specific locations, including extensions at the 5' and 3' termini. Comparison with secondary structure diagrams of 16S-like RNAs from several organisms allowed us to specify highly conserved and variable regions of this gene. Phylogenetic tree construction indicated that this gene is grouped with other mitochondrial genes, but most closely, as expected, with the fungal mitochondrial genes.

MeSH Terms
Ascomycota/genetics Base Sequence DNA, Fungal/genetics DNA, Mitochondrial/genetics,ultrastructure DNA, Ribosomal/genetics,ultrastructure Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation Phylogeny Sequence Homology, Nucleic Acid
Chemicals
DNA, Fungal DNA, Mitochondrial DNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cummings D J
Department of Microbiology and Immunology, University of Colorado School of Medicine, Denver 80262.
Domenico J M
Nelson J
Sogin M L
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02102481
Published
1989-03-00
Pages
232-41
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Grants
NIA NIH HHS · AG06320 · United States
NIGMS NIH HHS · GM32964 · United States
Databases
GENBANK
X14734
External Links
PubMed source DOI: 10.1007/BF02102481 View journal details
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