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PMID: 25005921 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

The embryo as a laboratory: quantifying transcription in Drosophila.

Trends in genetics : TIG ·Vol. 30 ·No. 8 ·2014-08-00 ·Pages 364-75

Gregor T, Garcia HG, Little SC

Abstract

Transcriptional regulation of gene expression is fundamental to most cellular processes, including determination of cellular fates. Quantitative studies of transcription in cultured cells have led to significant advances in identifying mechanisms underlying transcriptional control. Recent progress allowed implementation of these same quantitative methods in multicellular organisms to ask how transcriptional regulation unfolds both in vivo and at the single molecule level in the context of embryonic development. Here we review some of these advances in early Drosophila development, which bring the embryo on par with its single celled counterparts. In particular, we discuss progress in methods to measure mRNA and protein distributions in fixed and living embryos, and we highlight some initial applications that lead to fundamental new insights about molecular transcription processes. We end with an outlook on how to further exploit the unique advantages that come with investigating transcriptional control in the multicellular context of development.

Keywords
embryogenesis gene regulatory networks live imaging quantitative biology single molecule FISH
MeSH Terms
Animals Drosophila Proteins/genetics,metabolism Drosophila melanogaster/genetics,metabolism Embryo, Nonmammalian/cytology,metabolism Gene Expression Regulation, Developmental Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Drosophila Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gregor Thomas
Joseph Henry Laboratories of Physics, Princeton University, Princeton, NJ 085444, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA. Electronic address: [email protected].
Garcia Hernan G
Joseph Henry Laboratories of Physics, Princeton University, Princeton, NJ 085444, USA.
Little Shawn C
Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA.
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Article Info
Journal
Trends in genetics : TIG
Abbr.
Trends Genet
ISSN
0168-9525
DOI
10.1016/j.tig.2014.06.002
Email
[email protected]
Published
2014-08-00
Epub
2014-00-06
Pages
364-75
Language
English
Region
England
NLM ID
8507085
PMCID
PMC4129518
Subset
IM
Grants
NIGMS NIH HHS · P50 GM071508 · United States
NIGMS NIH HHS · R01 GM097275 · United States
External Links
PubMed source DOI: 10.1016/j.tig.2014.06.002 PMC Full Text View journal details
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