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PMID: 2508087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunoglobulin enhancer and promoter motifs 5' of the B29 B-cell-specific gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 19 ·1989-10-00 ·Pages 7341-5

Hermanson GG, Briskin M, Sigman D, Wall R

Abstract

B29 is a B-cell-specific member of the immunoglobulin gene superfamily that is expressed throughout B-cell development beginning with the earliest precursor B cells undergoing immunoglobulin heavy-chain gene segment rearrangements. We have analyzed the region upstream of the B29 gene to identify DNA sequences involved in transcriptional regulation of this gene. The B29 gene lacks a TATA box and transcription is initiated at multiple sites. The B29 gene sequence 5' of these transcription start sites contains six promoter and enhancer motifs known to control immunoglobulin gene transcription. The most notable is a perfect octamer (5'-ATTTGCAT-3'), which binds the Oct-2 B-cell-specific transcription factor and thereby can account for the tissue-specific expression of this gene.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Enhancer Elements, Genetic Genes, Immunoglobulin Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Molecular Sequence Data Multigene Family Nucleotide Mapping Oligonucleotide Probes Promoter Regions, Genetic RNA, Messenger/genetics Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region Oligonucleotide Probes RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hermanson G G
Molecular Biology Institute, University of California, Los Angeles 90024.
Briskin M
Sigman D
Wall R
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47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.19.7341
Published
1989-10-00
Pages
7341-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298057
Subset
IM
Grants
NCI NIH HHS · CA 12800 · United States
NIGMS NIH HHS · GM39559 · United States
NIGMS NIH HHS · GM40185 · United States
Databases
GENBANK
M26184
External Links
PubMed source DOI: 10.1073/pnas.86.19.7341 PMC Full Text View journal details
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