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PMID: 25280766 Published · epublish English Journal Article Review

Endocytic accessory factors and regulation of clathrin-mediated endocytosis.

Cold Spring Harbor perspectives in biology ·Vol. 6 ·No. 11 ·2014-10-03 ·Pages a016733

Merrifield CJ, Kaksonen M

Abstract

Up to 60 different proteins are recruited to the site of clathrin-mediated endocytosis in an ordered sequence. These accessory proteins have roles during all the different stages of clathrin-mediated endocytosis. First, they participate in the initiation of the endocytic event, thereby determining when and where endocytic vesicles are made; later they are involved in the maturation of the clathrin coat, recruitment of specific cargo molecules, bending of the membrane, and finally in scission and uncoating of the nascent vesicle. In addition, many of the accessory components are involved in regulating and coupling the actin cytoskeleton to the endocytic membrane. We will discuss the different accessory components and their various roles. Most of the data comes from studies performed with cultured mammalian cells or yeast cells. The process of endocytosis is well conserved between these different organisms, but there are also many interesting differences that may shed light on the mechanistic principles of endocytosis.

MeSH Terms
Animals Cell Membrane/metabolism,physiology Clathrin-Coated Vesicles/metabolism,physiology Endocytosis/physiology Mammals/metabolism Models, Biological Yeasts/cytology,metabolism
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Merrifield Christien J
Laboratoire d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique UPR3082, 91198 Gif-sur-Yvette, France.
Kaksonen Marko
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
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Article Info
Journal
Cold Spring Harbor perspectives in biology
Abbr.
Cold Spring Harb Perspect Biol
ISSN
1943-0264
DOI
10.1101/cshperspect.a016733
Published
2014-10-03
Epub
2014-00-03
Pages
a016733
Language
English
Region
United States
NLM ID
101513680
PMCID
PMC4413230
Subset
IM
Grants
Medical Research Council · MC_U105178789 · United Kingdom
External Links
PubMed source DOI: 10.1101/cshperspect.a016733 PMC Full Text View journal details
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