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PMID: 21779028 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Review

Molecular mechanism and physiological functions of clathrin-mediated endocytosis.

Nature reviews. Molecular cell biology ·Vol. 12 ·No. 8 ·2011-07-22 ·Pages 517-33

McMahon HT, Boucrot E

Abstract

Clathrin-mediated endocytosis is the endocytic portal into cells through which cargo is packaged into vesicles with the aid of a clathrin coat. It is fundamental to neurotransmission, signal transduction and the regulation of many plasma membrane activities and is thus essential to higher eukaryotic life. Morphological stages of vesicle formation are mirrored by progression through various protein modules (complexes). The process involves the formation of a putative FCH domain only (FCHO) initiation complex, which matures through adaptor protein 2 (AP2)-dependent cargo selection, and subsequent coat building, dynamin-mediated scission and finally auxilin- and heat shock cognate 70 (HSC70)-dependent uncoating. Some modules can be used in other pathways, and additions or substitutions confer cell specificity and adaptability.

MeSH Terms
Actins/physiology Adaptor Protein Complex 2/antagonists & inhibitors,genetics,physiology Adaptor Proteins, Vesicular Transport/physiology Animals Clathrin/antagonists & inhibitors,genetics,physiology Clathrin-Coated Vesicles/physiology Dynamins/physiology Endocytosis/physiology Humans Models, Biological Mutation Neoplasms/etiology RNA Interference Signal Transduction Synaptic Vesicles/physiology
Chemicals
Actins Adaptor Protein Complex 2 Adaptor Proteins, Vesicular Transport Clathrin Dynamins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McMahon Harvey T
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. [email protected]
Boucrot Emmanuel
References (182)
182 references, click to expand
  1. Endocytic mechanisms responsible for uptake of GPI-linked diphtheria toxin receptor.
    J Cell Sci. 1999 Nov;112 ( Pt 22):3899-909 PMID: 10547351
  2. Importance of the pleckstrin homology domain of dynamin in clathrin-mediated endocytosis.
    Curr Biol. 1999 Mar 11;9(5):257-60 PMID: 10074456
  3. Endosomal recycling controls plasma membrane area during mitosis.
    Proc Natl Acad Sci U S A. 2007 May 8;104(19):7939-44 PMID: 17483462
  4. Single-molecule analysis of a molecular disassemblase reveals the mechanism of Hsc70-driven clathrin uncoating.
    Nat Struct Mol Biol. 2011 Mar;18(3):295-301 PMID: 21278753
  5. Calcium triggers calcineurin-dependent synaptic vesicle recycling in mammalian nerve terminals.
    Curr Biol. 1998 Jun 18;8(13):740-9 PMID: 9651678
  6. Phenylarsine oxide inhibition of endocytosis: effects on asialofetuin internalization.
    Am J Physiol. 1989 Aug;257(2 Pt 1):C182-4 PMID: 2475026
  7. Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells.
    Nat Cell Biol. 2011 Mar;13(3):331-7 PMID: 21297641
  8. The length of vesicular stomatitis virus particles dictates a need for actin assembly during clathrin-dependent endocytosis.
    PLoS Pathog. 2010 Sep 30;6(9):e1001127 PMID: 20941355
  9. Structure and analysis of FCHo2 F-BAR domain: a dimerizing and membrane recruitment module that effects membrane curvature.
    Structure. 2007 Jul;15(7):839-52 PMID: 17540576
  10. Clathrin is spindle-associated but not essential for mitosis.
    PLoS One. 2008 Sep 03;3(9):e3115 PMID: 18769625
  11. Clathrin is required for the function of the mitotic spindle.
    Nature. 2005 Apr 28;434(7037):1152-7 PMID: 15858577
  12. Excessive expression of synaptojanin in brains with Down syndrome.
    Brain Dev. 2002 Mar;24(2):67-72 PMID: 11891094
  13. Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosis.
    Neuron. 1998 Dec;21(6):1465-75 PMID: 9883738
  14. Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein.
    Science. 2001 May 18;292(5520):1394-8 PMID: 11326085
  15. The endocytic matrix.
    Nature. 2010 Jan 28;463(7280):464-73 PMID: 20110990
  16. Role of beta-arrestin in mediating agonist-promoted G protein-coupled receptor internalization.
    Science. 1996 Jan 19;271(5247):363-6 PMID: 8553074
  17. A structural explanation for the binding of endocytic dileucine motifs by the AP2 complex.
    Nature. 2008 Dec 18;456(7224):976-979 PMID: 19140243
  18. Yolk transport in the ovarian follicle of the hen (Gallus domesticus): lipoprotein-like particles at the periphery of the oocyte in the rapid growth phase.
    J Cell Sci. 1979 Oct;39:257-72 PMID: 231041
  19. Clathrin-mediated internalization is essential for sustained EGFR signaling but dispensable for degradation.
    Dev Cell. 2008 Aug;15(2):209-19 PMID: 18694561
  20. Type II phosphatidylinositol 4-kinases promote Listeria monocytogenes entry into target cells.
    Cell Microbiol. 2007 Oct;9(10):2381-90 PMID: 17555516
  21. Dynasore, a cell-permeable inhibitor of dynamin.
    Dev Cell. 2006 Jun;10(6):839-50 PMID: 16740485
  22. Role of auxilin in uncoating clathrin-coated vesicles.
    Nature. 1995 Dec 7;378(6557):632-5 PMID: 8524399
  23. Amantadine and dansylcadaverine inhibit vesicular stomatitis virus uptake and receptor-mediated endocytosis of alpha 2-macroglobulin.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2291-5 PMID: 6179094
  24. The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.
    Proc Natl Acad Sci U S A. 1996 May 14;93(10):4804-9 PMID: 8643484
  25. The "vesicle in a basket". A morphological study of the coated vesicle isolated from the nerve endings of the guinea pig brain, with special reference to the mechanism of membrane movements.
    J Cell Biol. 1969 Jul;42(1):202-20 PMID: 4182372
  26. Phosphatidylinositol-(4,5)-bisphosphate regulates sorting signal recognition by the clathrin-associated adaptor complex AP2.
    Mol Cell. 2005 May 27;18(5):519-31 PMID: 15916959
  27. Adaptable adaptors for coated vesicles.
    Trends Cell Biol. 2004 Apr;14(4):167-74 PMID: 15066634
  28. Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin.
    J Cell Biol. 1995 Oct;131(1):69-80 PMID: 7559787
  29. Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation.
    J Cell Biol. 1993 Dec;123(5):1107-17 PMID: 8245121
  30. A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin.
    Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6927-32 PMID: 21482805
  31. Shiga toxin induces tubular membrane invaginations for its uptake into cells.
    Nature. 2007 Nov 29;450(7170):670-5 PMID: 18046403
  32. Pathways and mechanisms of endocytic recycling.
    Nat Rev Mol Cell Biol. 2009 Sep;10(9):597-608 PMID: 19696797
  33. The Drosophila clathrin heavy chain gene: clathrin function is essential in a multicellular organism.
    Genetics. 1993 Aug;134(4):1119-34 PMID: 8375651
  34. Mechanisms of endocytosis.
    Annu Rev Biochem. 2009;78:857-902 PMID: 19317650
  35. Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis.
    J Cell Biol. 2010 Mar 22;188(6):919-33 PMID: 20231386
  36. Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis.
    Curr Biol. 2007 Mar 20;17(6):520-7 PMID: 17306539
  37. The lipid phosphatase SHIP2 controls insulin sensitivity.
    Nature. 2001 Jan 4;409(6816):92-7 PMID: 11343120
  38. Membrane curvature controls dynamin polymerization.
    Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4141-6 PMID: 20160074
  39. ENTH domain proteins are cargo adaptors for multiple SNARE proteins at the TGN endosome.
    J Cell Sci. 2008 Feb 1;121(Pt 3):329-38 PMID: 18198191
  40. Nucleotide-dependent conformational changes in dynamin: evidence for a mechanochemical molecular spring.
    Nat Cell Biol. 1999 May;1(1):27-32 PMID: 10559860
  41. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors.
    Mol Biol Cell. 2006 Oct;17(10):4300-17 PMID: 16870701
  42. Distinct saturable pathways for the endocytosis of different tyrosine motifs.
    J Biol Chem. 1998 Jul 3;273(27):17056-63 PMID: 9642270
  43. THE DISTRIBUTION OF EXOGENOUS FERRITIN IN TOAD SPINAL GANGLIA AND THE MECHANISM OF ITS UPTAKE BY NEURONS.
    J Cell Biol. 1964 Nov;23:307-25 PMID: 14222816
  44. Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits.
    Dev Cell. 2009 Dec;17(6):811-22 PMID: 20059951
  45. Fast, three-dimensional super-resolution imaging of live cells.
    Nat Methods. 2011 Jun;8(6):499-508 PMID: 21552254
  46. Diverse somatic mutation patterns and pathway alterations in human cancers.
    Nature. 2010 Aug 12;466(7308):869-73 PMID: 20668451
  47. Essential role of phosphoinositide metabolism in synaptic vesicle recycling.
    Cell. 1999 Oct 15;99(2):179-88 PMID: 10535736
  48. Assembly of endocytic machinery around individual influenza viruses during viral entry.
    Nat Struct Mol Biol. 2004 Jun;11(6):567-73 PMID: 15122347
  49. Role of lipids and actin in the formation of clathrin-coated pits.
    Exp Cell Res. 2006 Dec 10;312(20):4036-48 PMID: 17097636
  50. Clathrin, AP-2, and the NPXY-binding subset of alternate endocytic adaptors facilitate FimH-mediated bacterial invasion of host cells.
    Cell Microbiol. 2008 Dec;10(12):2553-67 PMID: 18754852
  51. Vesicular stomatitis virus enters cells through vesicles incompletely coated with clathrin that depend upon actin for internalization.
    PLoS Pathog. 2009 Apr;5(4):e1000394 PMID: 19390604
  52. Association of Dishevelled with the clathrin AP-2 adaptor is required for Frizzled endocytosis and planar cell polarity signaling.
    Dev Cell. 2007 Jan;12(1):129-41 PMID: 17199046
  53. Invasive and adherent bacterial pathogens co-Opt host clathrin for infection.
    Cell Host Microbe. 2007 Nov 15;2(5):340-51 PMID: 18005755
  54. Candida albicans internalization by host cells is mediated by a clathrin-dependent mechanism.
    Cell Microbiol. 2009 Aug;11(8):1179-89 PMID: 19416270
  55. Coated pits act as molecular filters.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4156-9 PMID: 6968906
  56. Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation.
    J Cell Biol. 1989 Feb;108(2):389-400 PMID: 2563728
  57. A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis.
    PLoS Biol. 2011 Mar;9(3):e1000604 PMID: 21445324
  58. Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb.
    Cell. 2008 Sep 5;134(5):817-27 PMID: 18775314
  59. Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts.
    Cell. 1977 Mar;10(3):351-64 PMID: 191195
  60. Receptor-mediated entry of diphtheria toxin into monkey kidney (Vero) cells: electron microscopic evaluation.
    Infect Immun. 1985 Dec;50(3):721-7 PMID: 4066029
  61. Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells.
    J Cell Biol. 2010 Aug 23;190(4):675-91 PMID: 20713605
  62. Coated vesicles from the protozoan parasite Trypanosoma brucei: purification and characterization.
    J Protozool. 1989 Jul-Aug;36(4):344-9 PMID: 2570143
  63. YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L.
    J Cell Biol. 1964 Feb;20:313-32 PMID: 14126875
  64. Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly.
    J Mol Biol. 2007 Jan 19;365(3):892-9 PMID: 17095010
  65. Role of Drosophila alpha-adaptin in presynaptic vesicle recycling.
    Cell. 1997 Mar 21;88(6):767-76 PMID: 9118220
  66. Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
    PLoS Biol. 2009 Sep;7(9):e1000191 PMID: 19809571
  67. Mutations in dynamin 2 cause dominant centronuclear myopathy.
    Nat Genet. 2005 Nov;37(11):1207-9 PMID: 16227997
  68. Factors regulating the abundance and localization of synaptobrevin in the plasma membrane.
    Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11399-404 PMID: 16844789
  69. Stonin 2 is an AP-2-dependent endocytic sorting adaptor for synaptotagmin internalization and recycling.
    Dev Cell. 2006 Feb;10(2):233-44 PMID: 16459302
  70. Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling.
    Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3833-8 PMID: 15007177
  71. Identification of coated vesicles in Saccharomyces cerevisiae.
    J Cell Biol. 1984 Jan;98(1):341-6 PMID: 6368570
  72. Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating.
    Nature. 2004 Dec 2;432(7017):649-53 PMID: 15502813
  73. Numb is an endocytic protein.
    J Cell Biol. 2000 Dec 11;151(6):1345-52 PMID: 11121447
  74. BAR domains as sensors of membrane curvature: the amphiphysin BAR structure.
    Science. 2004 Jan 23;303(5657):495-9 PMID: 14645856
  75. Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction.
    J Cell Biol. 1973 May;57(2):315-44 PMID: 4348786
  76. A triggered mechanism retrieves membrane in seconds after Ca(2+)-stimulated exocytosis in single pituitary cells.
    J Cell Biol. 1994 Mar;124(5):667-75 PMID: 8120090
  77. Auxin: a trigger for change in plant development.
    Cell. 2009 Mar 20;136(6):1005-16 PMID: 19303845
  78. Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.
    PLoS Biol. 2006 Sep;4(9):e262 PMID: 16903783
  79. Saturation of the endocytic pathway for the transferrin receptor does not affect the endocytosis of the epidermal growth factor receptor.
    J Biol Chem. 1997 Jan 24;272(4):2116-21 PMID: 8999911
  80. Receptor-mediated endocytosis of transferrin and epidermal growth factor receptors: a comparison of constitutive and ligand-induced uptake.
    J Cell Sci Suppl. 1985;3:173-86 PMID: 3011821
  81. A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction.
    Mol Biol Cell. 2008 Jan;19(1):405-13 PMID: 17978091
  82. Multiple calcium-dependent processes related to secretion in bovine chromaffin cells.
    Neuron. 1993 Jan;10(1):21-30 PMID: 8427700
  83. Clathrin-mediated endocytosis in AP-2-depleted cells.
    J Cell Biol. 2003 Sep 1;162(5):909-18 PMID: 12952941
  84. Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila melanogaster, shibirets1.
    J Cell Biol. 1983 Aug;97(2):499-507 PMID: 6411734
  85. Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy.
    Nat Genet. 2007 Sep;39(9):1134-9 PMID: 17676042
  86. An endophilin-dynamin complex promotes budding of clathrin-coated vesicles during synaptic vesicle recycling.
    J Cell Sci. 2011 Jan 1;124(Pt 1):133-43 PMID: 21172823
  87. GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission.
    Nature. 2006 May 25;441(7092):528-31 PMID: 16648839
  88. Amphiphysin heterodimers: potential role in clathrin-mediated endocytosis.
    Mol Biol Cell. 1997 Oct;8(10):2003-15 PMID: 9348539
  89. Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells.
    J Cell Biol. 2003 Sep 29;162(7):1293-303 PMID: 14504267
  90. Bending a membrane: how clathrin affects budding.
    Proc Natl Acad Sci U S A. 2006 Jun 6;103(23):8715-20 PMID: 16735469
  91. Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria.
    Dev Cell. 2010 Feb 16;18(2):324-31 PMID: 20159602
  92. Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process.
    J Cell Biol. 2003 Feb 3;160(3):321-8 PMID: 12551953
  93. Interaction of tyrosine-based sorting signals with clathrin-associated proteins.
    Science. 1995 Sep 29;269(5232):1872-5 PMID: 7569928
  94. A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating.
    Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10265-10270 PMID: 16798879
  95. Endocytosis of the anthrax toxin is mediated by clathrin, actin and unconventional adaptors.
    PLoS Pathog. 2010 Mar 05;6(3):e1000792 PMID: 20221438
  96. FCHo proteins are nucleators of clathrin-mediated endocytosis.
    Science. 2010 Jun 4;328(5983):1281-4 PMID: 20448150
  97. Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles.
    J Med Chem. 2009 Jun 25;52(12):3762-73 PMID: 19459681
  98. Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin1.
    J Cell Biol. 2006 Jul 31;174(3):459-71 PMID: 16880274
  99. Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1255-9 PMID: 1063406
  100. Effects of dynamin inactivation on pathways of anthrax toxin uptake.
    Eur J Cell Biol. 2004 Jul;83(6):281-8 PMID: 15511085
  101. Endocytosis by random initiation and stabilization of clathrin-coated pits.
    Cell. 2004 Sep 3;118(5):591-605 PMID: 15339664
  102. Drosophila stoned proteins regulate the rate and fidelity of synaptic vesicle internalization.
    J Neurosci. 2001 May 1;21(9):3034-44 PMID: 11312288
  103. Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development.
    J Cell Biol. 2007 Jul 16;178(2):309-22 PMID: 17620409
  104. Mutations in human dynamin block an intermediate stage in coated vesicle formation.
    J Cell Biol. 1993 Aug;122(3):553-63 PMID: 8101525
  105. Initial events during phagocytosis by macrophages viewed from outside and inside the cell: membrane-particle interactions and clathrin.
    J Cell Biol. 1982 Sep;94(3):613-23 PMID: 6813339
  106. Hrs recruits clathrin to early endosomes.
    EMBO J. 2001 Sep 3;20(17):5008-21 PMID: 11532964
  107. Integrating molecular and network biology to decode endocytosis.
    Nature. 2007 Aug 23;448(7156):883-8 PMID: 17713526
  108. Embryonic arrest at midgestation and disruption of Notch signaling produced by the absence of both epsin 1 and epsin 2 in mice.
    Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13838-43 PMID: 19666558
  109. Clathrin-dependent targeting of receptors to the flagellar pocket of procyclic-form Trypanosoma brucei.
    Eukaryot Cell. 2004 Aug;3(4):1004-14 PMID: 15302833
  110. Clathrin-coated vesicles in nervous tissue are involved primarily in synaptic vesicle recycling.
    J Cell Biol. 1992 Sep;118(6):1379-88 PMID: 1325974
  111. AP-2 recruitment to synaptotagmin stimulated by tyrosine-based endocytic motifs.
    Science. 1999 Aug 20;285(5431):1268-71 PMID: 10455054
  112. Clathrin is a key regulator of basolateral polarity.
    Nature. 2008 Apr 10;452(7188):719-23 PMID: 18401403
  113. A modular design for the clathrin- and actin-mediated endocytosis machinery.
    Cell. 2005 Oct 21;123(2):305-20 PMID: 16239147
  114. A role for the dynamin-like protein Vps1 during endocytosis in yeast.
    J Cell Sci. 2010 Oct 15;123(Pt 20):3496-506 PMID: 20841380
  115. Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools.
    Proc Natl Acad Sci U S A. 2009 Jan 27;106(4):1139-44 PMID: 19151157
  116. Clathrin adaptor epsinR is required for retrograde sorting on early endosomal membranes.
    Dev Cell. 2004 Apr;6(4):525-38 PMID: 15068792
  117. Molecular anatomy of a trafficking organelle.
    Cell. 2006 Nov 17;127(4):831-46 PMID: 17110340
  118. Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes.
    Science. 2001 Feb 9;291(5506):1051-5 PMID: 11161218
  119. Early-arriving Syp1p and Ede1p function in endocytic site placement and formation in budding yeast.
    Mol Biol Cell. 2009 Nov;20(22):4640-51 PMID: 19776351
  120. Structural basis of membrane invagination by F-BAR domains.
    Cell. 2008 Mar 7;132(5):807-17 PMID: 18329367
  121. Inhibition of clathrin-coated pit assembly by an Eps15 mutant.
    J Cell Sci. 1999 May;112 ( Pt 9):1303-11 PMID: 10194409
  122. C. elegans Disabled is required for cell-type specific endocytosis and is essential in animals lacking the AP-3 adaptor complex.
    J Cell Sci. 2007 Aug 1;120(Pt 15):2741-51 PMID: 17636000
  123. Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release.
    Cell. 2002 Apr 5;109(1):101-12 PMID: 11955450
  124. Actin assembly plays a variable, but not obligatory role in receptor-mediated endocytosis in mammalian cells.
    Traffic. 2000 Feb;1(2):161-71 PMID: 11208096
  125. Clathrin heavy chain is required for pinocytosis, the presence of large vacuoles, and development in Dictyostelium.
    J Cell Biol. 1992 Sep;118(6):1371-7 PMID: 1522112
  126. A novel gene, AF-1p, fused to HRX in t(1;11)(p32;q23), is not related to AF-4, AF-9 nor ENL.
    Oncogene. 1994 Apr;9(4):1039-45 PMID: 8134107
  127. Eps15 is a component of clathrin-coated pits and vesicles and is located at the rim of coated pits.
    J Biol Chem. 1996 Nov 15;271(46):28727-30 PMID: 8910509
  128. GTPase cycle of dynamin is coupled to membrane squeeze and release, leading to spontaneous fission.
    Cell. 2008 Dec 26;135(7):1276-86 PMID: 19084269
  129. Endocytosis and signalling: intertwining molecular networks.
    Nat Rev Mol Cell Biol. 2009 Sep;10(9):609-22 PMID: 19696798
  130. Dap160/intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis.
    Neuron. 2004 Jul 22;43(2):193-205 PMID: 15260956
  131. Dynamin undergoes a GTP-dependent conformational change causing vesiculation.
    Cell. 1998 Jun 12;93(6):1021-9 PMID: 9635431
  132. Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):451-5 PMID: 6952195
  133. Depletion of intracellular potassium arrests coated pit formation and receptor-mediated endocytosis in fibroblasts.
    Cell. 1983 May;33(1):273-85 PMID: 6147196
  134. Identification of a beta-type adaptin in plant clathrin-coated vesicles.
    J Cell Sci. 1994 Apr;107 ( Pt 4):945-53 PMID: 8056848
  135. Endocytic adaptor molecules reveal an endosomal population of clathrin by total internal reflection fluorescence microscopy.
    J Biol Chem. 2004 Mar 26;279(13):13190-204 PMID: 14722064
  136. Tickets to ride: selecting cargo for clathrin-regulated internalization.
    Nat Rev Mol Cell Biol. 2009 Sep;10(9):583-96 PMID: 19696796
  137. Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake.
    J Cell Biol. 2008 Sep 8;182(5):1007-16 PMID: 18762582
  138. Differential requirements for actin during yeast and mammalian endocytosis.
    Nat Cell Biol. 2009 Aug;11(8):1039-42 PMID: 19597484
  139. Alu-splice cloning of human Intersectin (ITSN), a putative multivalent binding protein expressed in proliferating and differentiating neurons and overexpressed in Down syndrome.
    Eur J Hum Genet. 1999 Sep;7(6):704-12 PMID: 10482960
  140. Roles of AP-2 in clathrin-mediated endocytosis.
    PLoS One. 2010 May 12;5(5):e10597 PMID: 20485680
  141. Curvature of clathrin-coated pits driven by epsin.
    Nature. 2002 Sep 26;419(6905):361-6 PMID: 12353027
  142. An enzyme that removes clathrin coats: purification of an uncoating ATPase.
    J Cell Biol. 1984 Aug;99(2):723-33 PMID: 6146630
  143. Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission.
    Mol Biol Cell. 2009 Nov;20(22):4630-9 PMID: 19776347
  144. Regulation of synaptic vesicle recycling by complex formation between intersectin 1 and the clathrin adaptor complex AP2.
    Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4206-11 PMID: 20160082
  145. Clathrin-dependent and clathrin-independent retrieval of synaptic vesicles in retinal bipolar cells.
    Neuron. 2005 Jun 16;46(6):869-78 PMID: 15953416
  146. Clathrin adaptor AP-2 is essential for early embryonal development.
    Mol Cell Biol. 2005 Nov;25(21):9318-23 PMID: 16227583
  147. Controlled elimination of clathrin heavy-chain expression in DT40 lymphocytes.
    Science. 2002 Aug 30;297(5586):1521-5 PMID: 12202821
  148. Membrane curvature and mechanisms of dynamic cell membrane remodelling.
    Nature. 2005 Dec 1;438(7068):590-6 PMID: 16319878
  149. Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila.
    Genes Dev. 2001 Nov 15;15(22):2967-79 PMID: 11711432
  150. Cdk5 is essential for synaptic vesicle endocytosis.
    Nat Cell Biol. 2003 Aug;5(8):701-10 PMID: 12855954
  151. Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast.
    J Cell Biol. 1993 Dec;123(6 Pt 2):1707-16 PMID: 8276891
  152. Clathrin functions in the absence of heterotetrameric adaptors and AP180-related proteins in yeast.
    EMBO J. 1999 Jul 15;18(14):3897-908 PMID: 10406795
  153. E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells.
    Cell. 1996 Mar 22;84(6):923-32 PMID: 8601315
  154. Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells.
    J Cell Biol. 2009 Nov 30;187(5):733-47 PMID: 19951918
  155. Analysis of clathrin-mediated endocytosis of epidermal growth factor receptor by RNA interference.
    J Biol Chem. 2004 Apr 16;279(16):16657-61 PMID: 14985334
  156. Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions.
    Science. 1997 Apr 11;276(5310):259-63 PMID: 9092476
  157. Mutations in the pleckstrin homology domain of dynamin 2 cause dominant intermediate Charcot-Marie-Tooth disease.
    Nat Genet. 2005 Mar;37(3):289-94 PMID: 15731758
  158. Three-dimensional visualization of coated vesicle formation in fibroblasts.
    J Cell Biol. 1980 Mar;84(3):560-83 PMID: 6987244
  159. Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly.
    Dev Cell. 2006 Mar;10(3):329-42 PMID: 16516836
  160. Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo.
    Nat Cell Biol. 2001 Feb;3(2):215-9 PMID: 11175756
  161. Protein scaffolds in the coupling of synaptic exocytosis and endocytosis.
    Nat Rev Neurosci. 2011 Mar;12(3):127-38 PMID: 21304549
  162. Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding.
    Nature. 1995 Mar 9;374(6518):190-2 PMID: 7877694
  163. Transmitter glutamate release from isolated nerve terminals: evidence for biphasic release and triggering by localized Ca2+.
    J Neurochem. 1991 Jan;56(1):86-94 PMID: 1670958
  164. The yeast dynamin-like protein Vps1:vps1 mutations perturb the internalization and the motility of endocytic vesicles and endosomes via disorganization of the actin cytoskeleton.
    Eur J Cell Biol. 2010 Jul;89(7):499-508 PMID: 20189679
  165. Endocytosis from coated pits of Shiga toxin: a glycolipid-binding protein from Shigella dysenteriae 1.
    J Cell Biol. 1989 Apr;108(4):1331-43 PMID: 2564398
  166. Clathrin-mediated endocytic signals are required for the regeneration of, as well as homeostasis in, the planarian CNS.
    Development. 2007 May;134(9):1679-89 PMID: 17376807
  167. Synaptojanin 1-mediated PI(4,5)P2 hydrolysis is modulated by membrane curvature and facilitates membrane fission.
    Dev Cell. 2011 Feb 15;20(2):206-18 PMID: 21316588
  168. Role of the human transferrin receptor cytoplasmic domain in endocytosis: localization of a specific signal sequence for internalization.
    J Cell Biol. 1990 Feb;110(2):283-94 PMID: 2298808
  169. Role of phosphorylation in regulation of the assembly of endocytic coat complexes.
    Science. 1998 Aug 7;281(5378):821-4 PMID: 9694653
  170. AP-1 and ARF1 control endosomal dynamics at sites of FcR mediated phagocytosis.
    Mol Biol Cell. 2007 Dec;18(12):4921-31 PMID: 17914058
  171. Listeria hijacks the clathrin-dependent endocytic machinery to invade mammalian cells.
    Nat Cell Biol. 2005 Sep;7(9):894-900 PMID: 16113677
  172. Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation.
    EMBO J. 2009 Oct 21;28(20):3103-16 PMID: 19713939
  173. Cargo regulates clathrin-coated pit dynamics.
    Cell. 2006 Oct 6;127(1):113-24 PMID: 17018281
  174. A role for clathrin in the sorting of vacuolar proteins in the Golgi complex of yeast.
    EMBO J. 1992 Aug;11(8):2811-8 PMID: 1639056
  175. Multiple interactions of auxilin 1 with clathrin and the AP-2 adaptor complex.
    J Biol Chem. 2001 Sep 28;276(39):36131-8 PMID: 11470803
  176. Structure of clathrin coat with bound Hsc70 and auxilin: mechanism of Hsc70-facilitated disassembly.
    EMBO J. 2010 Feb 3;29(3):655-65 PMID: 20033059
  177. Molecular architecture and functional model of the endocytic AP2 complex.
    Cell. 2002 May 17;109(4):523-35 PMID: 12086608
  178. Systematic sequencing of renal carcinoma reveals inactivation of histone modifying genes.
    Nature. 2010 Jan 21;463(7279):360-3 PMID: 20054297
  179. UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles.
    Mol Biol Cell. 1999 Jul;10(7):2343-60 PMID: 10397769
  180. Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2.
    J Cell Biol. 2007 Dec 31;179(7):1497-510 PMID: 18166656
  181. Low cytoplasmic pH inhibits endocytosis and transport from the trans-Golgi network to the cell surface.
    J Cell Biol. 1989 Feb;108(2):377-87 PMID: 2918022
  182. Clathrin-mediated endocytosis is essential in Trypanosoma brucei.
    EMBO J. 2003 Oct 1;22(19):4991-5002 PMID: 14517238
Article Info
Journal
Nature reviews. Molecular cell biology
Abbr.
Nat Rev Mol Cell Biol
ISSN
1471-0080
DOI
10.1038/nrm3151
Email
[email protected]
Published
2011-07-22
Epub
2011-00-22
Pages
517-33
Language
English
Region
England
NLM ID
100962782
Subset
IM
Grants
Medical Research Council · MC_U105178795 · United Kingdom
External Links
PubMed source DOI: 10.1038/nrm3151 View journal details
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