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PMID: 2583098 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Excision products of the T cell receptor gene support a progressive rearrangement model of the alpha/delta locus.

The EMBO journal ·Vol. 8 ·No. 11 ·1989-11-00 ·Pages 3261-70

Takeshita S, Toda M, Yamagishi H

Abstract

We have cloned extrachromosomal circular DNAs containing T cell receptor (TCR) delta gene segments in adult mouse thymocytes and splenocytes. We find that the frequency of circular DNA clones carrying germline delta sequences is lower than that of J alpha probe-positive clones, possibly related to increasing 5' distance from the most upstream J alpha segment. This suggests that the TCR alpha/delta locus is successively rearranged from within and that the delta-containing excision products are progressively diluted out by the subsequent cell division which includes further alpha gene rearrangements. In addition, examination of delta gene excision products revealed newly identified V delta subfamilies, the reciprocal joining of two D delta elements, J delta 2 usage in thymocytes and novel sequences homologous to the human delta-gene deleting elements.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA, Circular/genetics Gene Rearrangement, T-Lymphocyte Humans Mice Mice, Inbred BALB C Mice, Inbred C57BL Models, Genetic Molecular Sequence Data Receptors, Antigen, T-Cell/genetics Restriction Mapping Sequence Homology, Nucleic Acid Spleen/metabolism Thymus Gland/metabolism
Chemicals
DNA, Circular Receptors, Antigen, T-Cell
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takeshita S
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Toda M
Yamagishi H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-11-00
Pages
3261-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401453
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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