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PMID: 2722752 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication origins of single-stranded-DNA plasmid pUB110.

Journal of bacteriology ·Vol. 171 ·No. 6 ·1989-06-00 ·Pages 3366-72

Boe L, Gros MF, te Riele H, Ehrlich SD, Gruss A

Abstract

The two replication origins of plasmid pUB110 have been characterized. The site of initiation of DNA replication at the plus origin was mapped to within an 8-base-pair sequence. DNA synthesis initiated at the origin was made to terminate precociously in an inserted sequence of 18 base pairs that is homologous to a sequence in the origin. This suggests that pUB110 replicates as a rolling circle. The minus origin of plasmid pUB110 has been characterized, and the minimal sequence required for function has been determined. As with other minus origins, activity is orientation specific with respect to the direction of replication. Its activity is sensitive to rifampin in vivo, suggesting that RNA polymerase catalyzes single-strand to double-strand conversion. Unlike all other plasmids of gram-positive bacteria thus far described, the pUB110 minus origin is functional in more than one host.

MeSH Terms
Base Sequence DNA Mutational Analysis DNA Replication DNA, Single-Stranded/genetics DNA-Directed RNA Polymerases/physiology Molecular Sequence Data Plasmids Regulatory Sequences, Nucleic Acid Restriction Mapping Staphylococcus aureus/genetics
Chemicals
DNA, Single-Stranded DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Boe L
Laboratoire de Génétique Microbienne, INRA-Domaine de Vilvert, Jouy en Josas, France.
Gros M F
te Riele H
Ehrlich S D
Gruss A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.6.3366-3372.1989
Published
1989-06-00
Pages
3366-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210059
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.6.3366-3372.1989 PMC Full Text View journal details
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