Home LiteratureArticle Details
PMID: 3009863 Published · ppublish English Journal Article

Properties of intracellular bovine papillomavirus chromatin.

Journal of virology ·Vol. 58 ·No. 2 ·1986-05-00 ·Pages 500-7

Rösl F, Waldeck W, Zentgraf H, Sauer G

Abstract

Episomal nucleoprotein complexes of bovine papillomavirus type 1 (BPV-1) in transformed cells were exposed to DNase I treatment to localize hypersensitive regions. Such regions, which are indicative for gene expression, were found within the noncoding part of the genome, coinciding with the origin of replication and the 5' ends of most of the early mRNAs. However, there were also regions of hypersensitivity within the structural genes. These intragenic perturbations of the chromatin structure coincide with regulatory sequences at the DNA level. One of these regions maps in close proximity to a Z-DNA antibody-binding site which is located near the putative BPV-1 enhancer sequence.

MeSH Terms
Animals Antibodies, Monoclonal Bovine papillomavirus 1/genetics Cell Line Chromatin/analysis DNA Replication DNA, Viral/biosynthesis,genetics,immunology Deoxyribonuclease I/pharmacology Enhancer Elements, Genetic Genes Genes, Regulator Genes, Viral Mice Papillomaviridae/genetics RNA, Messenger/genetics RNA, Viral/genetics
Chemicals
Antibodies, Monoclonal Chromatin DNA, Viral RNA, Messenger RNA, Viral Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rösl F
Waldeck W
Zentgraf H
Sauer G
References (49)
49 references, click to expand
  1. Isolation of episomal bovine papillomavirus chromatin and identification of a DNase I-hypersensitive region.
    J Virol. 1983 May;46(2):567-74 PMID: 6302320
  2. DNAase I-hypersensitive sites of chromatin.
    Cell. 1981 Dec;27(3 Pt 2):413-5 PMID: 6101195
  3. Negatively supercoiled simian virus 40 DNA contains Z-DNA segments within transcriptional enhancer sequences.
    Nature. 1983 Jun 23-29;303(5919):674-9 PMID: 6304535
  4. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  5. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  6. Bovine papilloma virus contains an activator of gene expression at the distal end of the early transcription unit.
    Mol Cell Biol. 1983 Jun;3(6):1108-22 PMID: 6308425
  7. Morphological studies of transcription.
    Acta Endocrinol Suppl (Copenh). 1972;168:155-77 PMID: 4562652
  8. Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.
    J Virol. 1975 Feb;15(2):259-67 PMID: 163354
  9. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  10. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  11. Heterogeneity of spacer lengths in circles of amplified ribosomal DNA of two insect species, Dytiscus marginalis and Acheta domesticus.
    J Mol Biol. 1976 Dec;108(2):453-70 PMID: 1034708
  12. Simplified quantitative electron microscopy of biopolymers.
    Biopolymers. 1970;9(3):373-9 PMID: 5417647
  13. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  14. Higher order structure of simian virus 40 chromatin.
    Science. 1978 Aug 4;201(4354):406-15 PMID: 208155
  15. Characterization of a DNA-protein complex and capsomere subunits derived from polyoma virus by treatment with ethyleneglycol-bis-N,N'-tetraacetic acid and dithiothreitol.
    J Virol. 1978 Jul;27(1):193-204 PMID: 211250
  16. Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells.
    J Virol. 1979 Feb;29(2):612-23 PMID: 219250
  17. Deoxyribonuclease I sensitivity of the nontranscribed sequences flanking the 5' and 3' ends of the ovomucoid gene and the ovalbumin and its related X and Y genes in hen oviduct nuclei.
    Biochemistry. 1980 Sep 16;19(19):4403-41 PMID: 6250584
  18. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  19. In vitro tumorigenic transformation by a defined sub-genomic fragment of bovine papilloma virus DNA.
    Nature. 1980 Sep 4;287(5777):72-4 PMID: 6251381
  20. Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA.
    J Virol. 1980 Sep;35(3):962-4 PMID: 6252350
  21. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  22. Left-handed DNA in restriction fragments and a recombinant plasmid.
    Nature. 1981 Apr 23;290(5808):672-7 PMID: 6261149
  23. Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2727-31 PMID: 6265905
  24. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
    Nature. 1981 Sep 3;293(5827):79-81 PMID: 6267479
  25. Simian virus 40 maturation: chromatin modifications increase the accessibility of viral DNA to nuclease and RNA polymerase.
    J Virol. 1981 Aug;39(2):603-11 PMID: 6268846
  26. Virus-specific transcription in bovine papillomavirus-transformed mouse cells.
    Virology. 1982 May;119(1):22-34 PMID: 6280384
  27. Active chromatin.
    Nature. 1982 May 27;297(5864):289-95 PMID: 6210847
  28. Effect of the B--Z transition in poly(dG-m5dC) . poly(dG-m5dC) on nucleosome formation.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1771-5 PMID: 6952228
  29. Temperature-sensitive changes in the structure of globin chromatin in lines of red cell precursors transformed by ts-AEV.
    Cell. 1982 Apr;28(4):931-40 PMID: 7094019
  30. Bovine papilloma virus transcription: polyadenylated RNA species and assessment of the direction of transcription.
    J Virol. 1982 Jul;43(1):59-66 PMID: 6180175
  31. The primary structure and genetic organization of the bovine papillomavirus type 1 genome.
    Nature. 1982 Oct 7;299(5883):529-34 PMID: 6289124
  32. Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.
    Cell. 1982 Dec;31(2 Pt 1):309-18 PMID: 7159926
  33. Transcriptional organization of bovine papillomavirus type 1.
    J Virol. 1983 Sep;47(3):516-28 PMID: 6137574
  34. Host-specificities of papillomavirus, Moloney murine sarcoma virus and simian virus 40 enhancer sequences.
    EMBO J. 1983;2(7):1193-9 PMID: 6313355
  35. DNase I hypersensitive sites in the chromatin of human mu immunoglobulin heavy-chain genes.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):809-12 PMID: 6419125
  36. Characterization of the bovine papilloma virus plasmid maintenance sequences.
    Cell. 1984 Feb;36(2):391-401 PMID: 6319020
  37. Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9 PMID: 6320177
  38. A close association between sites of DNase I hypersensitivity and sites of enhanced cleavage by micrococcal nuclease in the 5'-flanking region of the actively transcribed ovalbumin gene.
    EMBO J. 1984 May;3(5):1137-44 PMID: 6329739
  39. A negative regulatory sequence near the mouse beta-maj globin gene associated with a region of potential Z-DNA.
    EMBO J. 1984 Jun;3(6):1263-72 PMID: 6086313
  40. Inhibition of eukaryotic tRNA transcription by potential Z-DNA sequences.
    EMBO J. 1984 Jul;3(7):1553-9 PMID: 6745243
  41. Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
    Cell. 1983 May;33(1):65-76 PMID: 6088056
  42. Origin of replication in episomal bovine papilloma virus type 1 DNA isolated from transformed cells.
    EMBO J. 1984 Sep;3(9):2173-8 PMID: 6092063
  43. tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene.
    Mol Cell Biol. 1984 Sep;4(9):1777-84 PMID: 6493233
  44. Transcriptional block caused by a negative supercoiling induced structural change in an alternating CG sequence.
    Cell. 1985 Jan;40(1):129-37 PMID: 2981624
  45. The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):699-702 PMID: 2983311
  46. Bovine papillomavirus contains multiple transforming genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1030-4 PMID: 2983327
  47. Messenger RNAs from the transforming region of bovine papilloma virus type I.
    J Mol Biol. 1985 Apr 20;182(4):541-54 PMID: 2989533
  48. Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product.
    Cell. 1985 Aug;42(1):183-91 PMID: 2990724
  49. Transcriptional control signals in the genome of bovine papillomavirus type 1.
    Nature. 1983 May 5-11;303(5912):77-80 PMID: 6302515
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.2.500-507.1986
Published
1986-05-00
Pages
500-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252937
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.58.2.500-507.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2