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PMID: 3011809 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane.

The Journal of cell biology ·Vol. 102 ·No. 6 ·1986-06-00 ·Pages 2147-57

Puddington L, Machamer CE, Rose JK

Abstract

Oligonucleotide-directed mutagenesis was used to construct chimeric cDNAs that encode the extracellular and transmembrane domains of the vesicular stomatitis virus glycoprotein (G) linked to the cytoplasmic domain of either the immunoglobulin mu membrane heavy chain, the hemagglutinin glycoprotein of influenza virus, or the small glycoprotein (p23) of infectious bronchitis virus. Biochemical analyses and immunofluorescence microscopy demonstrated that these hybrid genes were correctly expressed in eukaryotic cells and that the hybrid proteins were transported to the plasma membrane. The rate of transport to the Golgi complex of G protein with an immunoglobulin mu membrane cytoplasmic domain was approximately sixfold slower than G protein with its normal cytoplasmic domain. However, this rate was virtually identical to the rate of transport of micron heavy chain molecules measured in the B cell line WEHI 231. The rate of transport of G protein with a hemagglutinin cytoplasmic domain was threefold slower than wild type G protein and G protein with a p23 cytoplasmic domain, which were transported at similar rates. The combined results underscore the importance of the amino acid sequence in the cytoplasmic domain for efficient transport of G protein to the cell surface. Also, normal cytoplasmic domains from other transmembrane glycoproteins can substitute for the G protein cytoplasmic domain in transport of G protein to the plasma membrane. The method of constructing precise hybrid proteins described here will be useful in defining functions of specific domains of viral and cellular integral membrane proteins.

MeSH Terms
B-Lymphocytes/metabolism Biological Transport, Active Cell Line Cell Membrane/metabolism Coronaviridae/metabolism Cytoplasm/metabolism Genes, Viral Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/metabolism Humans Immunoglobulin mu-Chains/metabolism Membrane Glycoproteins Membrane Proteins/genetics,metabolism Oligonucleotides/genetics,metabolism Transfection Vesicular stomatitis Indiana virus/genetics,metabolism Viral Envelope Proteins Viral Proteins/genetics,metabolism
Chemicals
G protein, vesicular stomatitis virus Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Immunoglobulin mu-Chains Membrane Glycoproteins Membrane Proteins Oligonucleotides Viral Envelope Proteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Puddington L
Machamer C E
Rose J K
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.102.6.2147
Published
1986-06-00
Pages
2147-57
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114239
Subset
IM
Grants
NIAID NIH HHS · AI-15481 · United States
NCI NIH HHS · CA-14195 · United States
NIGMS NIH HHS · GM-33840 · United States
External Links
PubMed source DOI: 10.1083/jcb.102.6.2147 PMC Full Text View journal details
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