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PMID: 30190490 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Lysine acetylation modulates mouse sperm capacitation.

Scientific reports ·Vol. 8 ·No. 1 ·2018-00-06 ·Pages 13334

Ritagliati C, Luque GM, Stival C, Baro Graf C, Buffone MG, Krapf D

Abstract

Mammalian sperm are unable to fertilize the egg immediately after ejaculation. To gain fertilization competence, they need to undergo a series of modifications inside the female reproductive tract, known as capacitation. Capacitation involves several molecular events such as phosphorylation cascades, hyperpolarization of the plasma membrane and intracellular Ca2+ changes, which prepare the sperm to develop two essential features for fertilization competence: hyperactivation and acrosome reaction. Since sperm cells lack new protein biosynthesis, post-translational modification of existing proteins plays a crucial role to obtain full functionality. Here, we show the presence of acetylated proteins in murine sperm, which increase during capacitation. Pharmacological hyperacetylation of lysine residues in non-capacitated sperm induces activation of PKA, hyperpolarization of the sperm plasma membrane, CatSper opening and Ca2+ influx, all capacitation-associated molecular events. Furthermore, hyperacetylation of non-capacitated sperm promotes hyperactivation and prepares the sperm to undergo acrosome reaction. Together, these results indicate that acetylation could be involved in the acquisition of fertilization competence of mammalian sperm.

MeSH Terms
Acetylation Acrosome Reaction/physiology Animals Lysine/metabolism Male Mice Sperm Capacitation/physiology Spermatozoa/cytology,metabolism
Chemicals
Lysine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ritagliati Carla
Laboratory of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET-UNR, Rosario, 2000, Argentina.
Luque Guillermina M
Laboratory of Cellular and Molecular Reproductive Biology, Instituto de Biología y Medicina Experimental (IBYME), CONICET, Buenos Aires, C1428ADN, Argentina.
Stival Cintia
Laboratory of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET-UNR, Rosario, 2000, Argentina.
Baro Graf Carolina
Laboratory of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET-UNR, Rosario, 2000, Argentina.
Buffone Mariano G
Laboratory of Cellular and Molecular Reproductive Biology, Instituto de Biología y Medicina Experimental (IBYME), CONICET, Buenos Aires, C1428ADN, Argentina.
Krapf Dario
Laboratory of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET-UNR, Rosario, 2000, Argentina. [email protected].
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Article Info
Journal
Scientific reports
Abbr.
Sci Rep
ISSN
2045-2322
DOI
10.1038/s41598-018-31557-5
Email
[email protected]
Published
2018-00-06
Epub
2018-00-06
Pages
13334
Language
English
Region
England
NLM ID
101563288
PMCID
PMC6127136
Subset
IM
Grants
FIC NIH HHS · R01 TW008662 · United States
Ministry of Science, Technology and Productive Innovation, Argentina · Agencia Nacional de Promoci&amp
#x00F3
n Cient&amp
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fica y Tecnol&amp
#x00F3
gica (National Agency for Science and Technology, Argentina) · PICT 2014-2702 · International
Ministry of Science, Technology and Productive Innovation, Argentina · Agencia Nacional de Promoci&amp
#x00F3
n Cient&amp
#x00ED
fica y Tecnol&amp
#x00F3
gica (National Agency for Science and Technology, Argentina) · PICT 2015-3164 · International
External Links
PubMed source DOI: 10.1038/s41598-018-31557-5 PMC Full Text View journal details
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