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PMID: 3019237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis by using DNA probes of the OXA-1 beta-lactamase gene and its transposon.

Antimicrobial agents and chemotherapy ·Vol. 30 ·No. 1 ·1986-07-00 ·Pages 46-51

Ouellette M, Roy PH

Abstract

From recombinant clone pTY27, encoding an OXA-1 beta-lactamase gene, we performed subcloning experiments to more precisely delimit the gene. We describe the use as probes of six different restriction fragments known from subcloning experiments to be within the structural gene or part of the transposable element, Tn2603, flanking the OXA-1 determinant. We showed that the OXA-1 structural gene is slightly related to the OXA-2 determinant and also that sequences within Tn2603 are common to all the OXA- and PSE-producing strains tested. For epidemiological purposes, we began nucleotide sequencing of the OXA-1 determinant, and from preliminary sequence data we synthesized an oligonucleotide 15 bases in length, corresponding to a sequence within the OXA-1 gene. This oligonucleotide was found to be specific for the OXA-1 determinant, because it hybridized only to bacteria producing that beta-lactamase.

MeSH Terms
Cloning, Molecular DNA Transposable Elements DNA, Bacterial/analysis Genes, Bacterial Nucleic Acid Hybridization Plasmids beta-Lactamases/biosynthesis,genetics
Chemicals
DNA Transposable Elements DNA, Bacterial beta-lactamase OXA-2 beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ouellette M
Roy P H
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32 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.30.1.46
Published
1986-07-00
Pages
46-51
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC176433
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.30.1.46 PMC Full Text View journal details
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