Home LiteratureArticle Details
PMID: 3033311 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the Epstein-Barr virus gp350/220 gene in rodent and primate cells.

Journal of virology ·Vol. 61 ·No. 6 ·1987-06-00 ·Pages 1796-807

Whang Y, Silberklang M, Morgan A, Munshi S, Lenny AB, Ellis RW, Kieff E

Abstract

The gene encoding the Epstein-Barr virus envelope glycoproteins gp350 and gp220 was inserted downstream of the cytomegalovirus immediate-early, Moloney murine leukemia virus, mouse mammary tumor virus, or varicella-zoster virus gpI promoters in vectors containing selectable markers. Host cell and recombinant vector systems were defined which enabled the isolation of rodent or primate cell clones which expressed gp350/220 in substantial quantities. Continued expression of gp350/220 required maintenance of cells under positive selection for linked markers and periodic cloning. gp350/220 expressed in various host cells varied slightly in electrophoretic mobility, probably reflecting differences in glycosylation. Insertion of a stop codon into the gp350/220 open reading frame, upstream of the putative membrane anchor sequence, resulted in efficient secretion of truncated gp350 and gp220 from rat pituitary (GH3) cells. gp350/220 expressed in mammalian cells is highly immunogenic and elicits virus-neutralizing antibodies when administered to mice.

MeSH Terms
Animals Antibodies, Viral/immunology Antigens, Viral/biosynthesis Cell Line Cricetinae Cricetulus Female Fibroblasts Genetic Vectors Herpesvirus 4, Human/genetics,immunology Ovary Pituitary Gland Promoter Regions, Genetic Rats Recombinant Fusion Proteins/biosynthesis,genetics,immunology Recombinant Proteins/biosynthesis Species Specificity Vero Cells Viral Matrix Proteins
Chemicals
Antibodies, Viral Antigens, Viral EBV-associated membrane antigen, Epstein-Barr virus Recombinant Fusion Proteins Recombinant Proteins Viral Matrix Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Whang Y
Silberklang M
Morgan A
Munshi S
Lenny A B
Ellis R W
Kieff E
References (58)
58 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Synthesis and processing of the three major envelope glycoproteins of Epstein-Barr virus.
    J Virol. 1983 May;46(2):547-56 PMID: 6302318
  3. Proteins of Epstein-Barr Virus. II. Electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virus.
    J Virol. 1976 Apr;18(1):289-97 PMID: 176465
  4. Empirical predictions of protein conformation.
    Annu Rev Biochem. 1978;47:251-76 PMID: 354496
  5. Epidemiological evidence for causal relationship between Epstein-Barr virus and Burkitt's lymphoma from Ugandan prospective study.
    Nature. 1978 Aug 24;274(5673):756-61 PMID: 210392
  6. Characterization of cross-reacting antigens on the Epstein-Barr virus envelope and plasma membranes of producer cells.
    Cell. 1979 Jan;16(1):33-42 PMID: 217544
  7. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  8. Cross-neutralization of infectious mononucleosis and Burkitt lymphoma strains of Epstein-Barr virus with hyperimmune rabbit antisera.
    J Immunol. 1979 Jul;123(1):232-8 PMID: 221591
  9. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  10. Glycosylation pathways of two major Epstein-Barr virus membrane antigens.
    Virology. 1983 May;127(1):168-76 PMID: 6305014
  11. A structural investigation of the Epstein-Barr (EB) virus membrane antigen glycoprotein, gp340.
    J Gen Virol. 1984 Feb;65 ( Pt 2):397-404 PMID: 6319581
  12. An Epstein-Barr virus DNA fragment encodes messages for the two major envelope glycoproteins (gp350/300 and gp220/200).
    J Virol. 1984 Feb;49(2):413-7 PMID: 6319741
  13. DNA sequence of the US component of the varicella-zoster virus genome.
    EMBO J. 1983;2(12):2203-9 PMID: 6321154
  14. Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):659-63 PMID: 6322160
  15. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  16. Transcription and DNA sequence of the BamHI L fragment of B95-8 Epstein-Barr virus.
    EMBO J. 1984 May;3(5):1083-90 PMID: 6203743
  17. Construction and applications of a highly transmissible murine retrovirus shuttle vector.
    Cell. 1984 Jul;37(3):1053-62 PMID: 6331674
  18. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  19. Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4510-4 PMID: 6087328
  20. Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC.
    J Virol. 1985 Jan;53(1):81-8 PMID: 2981365
  21. The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes.
    Cell. 1985 Feb;40(2):237-46 PMID: 3871356
  22. Two major outer envelope glycoproteins of Epstein-Barr virus are encoded by the same gene.
    J Virol. 1985 Jun;54(3):665-74 PMID: 2987520
  23. Identification and characterization of the Epstein-Barr virus receptor on human B lymphocytes and its relationship to the C3d complement receptor (CR2).
    J Virol. 1985 Aug;55(2):347-51 PMID: 2410629
  24. Assembly of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
  25. Growth hormone gene expression in eukaryotic cells directed by the Rous sarcoma virus long terminal repeat or cytomegalovirus immediate-early promoter.
    Gene. 1985;38(1-3):227-32 PMID: 2998944
  26. Protection of cottontop tamarins against Epstein-Barr virus-induced malignant lymphoma by a prototype subunit vaccine.
    Nature. 1985 Nov 21-27;318(6043):287-9 PMID: 2999604
  27. Recombinant vaccinia virus induces neutralising antibodies in rabbits against Epstein-Barr virus membrane antigen gp340.
    EMBO J. 1985 Dec 1;4(12):3229-34 PMID: 3004944
  28. Not all potently neutralizing, vaccine-induced antibodies to Epstein-Barr virus ensure protection of susceptible experimental animals.
    Clin Exp Immunol. 1986 Mar;63(3):485-90 PMID: 3011324
  29. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.
    Endocrinology. 1968 Feb;82(2):342-52 PMID: 4951281
  30. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.
    Mol Cell Biol. 1981 Sep;1(9):854-64 PMID: 9279398
  31. Correlation between Epstein-Barr virus membrane antigen and three large cell surface glycoproteins.
    J Virol. 1979 Dec;32(3):885-94 PMID: 92577
  32. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  33. Radioimmune precipitation study comparing the Epstein-Barr virus membrane antigens expressed on P3HR-1 virus-superinfected Raji cells to those expressed on cells in a B-95 virus-transformed producer culture activated with tumor-promoting agent (TPA).
    Virology. 1980 Apr 30;102(2):360-9 PMID: 6245514
  34. Epstein-Barr virus-induced proteins. II. Analysis of surface polypeptides from EBV-producing and -superinfected cells by immunoprecipitation.
    Virology. 1980 Apr 30;102(2):401-11 PMID: 6154378
  35. Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2979-83 PMID: 6248876
  36. Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003 PMID: 6248879
  37. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4216-20 PMID: 6933469
  38. Monoclonal antibodies against the major glycoprotein (gp350/220) of Epstein-Barr virus neutralize infectivity.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5307-11 PMID: 6254073
  39. Observations on the EB virus envelope and virus-determined membrane antigen (MA) polypeptides.
    Int J Cancer. 1980 Aug;26(2):231-40 PMID: 6162806
  40. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  41. The large-scale cultivation of VERO cells in micro-carrier culture for virus vaccine production. Preliminary results for killed poliovirus vaccine.
    Dev Biol Stand. 1981;47:55-64 PMID: 6785126
  42. Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1930-4 PMID: 6112750
  43. Epstein-Barr virus DNA. IX. Variation among viral DNAs from producer and nonproducer infected cells.
    J Virol. 1981 May;38(2):632-48 PMID: 6264134
  44. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6 PMID: 7017722
  45. Detection of antibodies and antigenemia in leukemic patients with candidiasis by enzyme-linked immunosorbent assay.
    J Infect Dis. 1981 Jul;144(1):24-32 PMID: 7021699
  46. Epstein-Barr virus membrane antigens: characterization, distribution, and strain differences.
    J Virol. 1981 Jul;39(1):172-84 PMID: 6268807
  47. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  48. A new dominant hybrid selective marker for higher eukaryotic cells.
    J Mol Biol. 1981 Jul 25;150(1):1-14 PMID: 6271971
  49. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.
    Nature. 1981 Nov 19;294(5838):228-32 PMID: 6272123
  50. Purification and biologic characterization of a major Epstein Barr virus-induced membrane glycoprotein.
    J Immunol. 1982 Aug;129(2):814-8 PMID: 7086144
  51. Production and characterization of monoclonal antibodies against the Epstein-Barr virus membrane antigen.
    J Virol. 1982 Jan;41(1):258-64 PMID: 6177865
  52. Two large virion envelope glycoproteins mediate Epstein-Barr virus binding to receptor-positive cells.
    J Virol. 1982 Jan;41(1):286-97 PMID: 6283120
  53. Identification and isolation of the main component (gp350-gp220) of Epstein-Barr virus responsible for generating neutralizing antibodies in vivo.
    J Virol. 1982 Aug;43(2):730-6 PMID: 6287039
  54. Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4030-4 PMID: 6287462
  55. Induction of thymidine kinase in enzyme-deficient Chinese hamster cells.
    Cell. 1982 Jun;29(2):483-92 PMID: 6180833
  56. Cloning and nucleotide sequencing of the bovine growth hormone gene.
    Nucleic Acids Res. 1982 Nov 25;10(22):7197-210 PMID: 6296767
  57. Purification and properties of the gp340 component of Epstein-Barr virus membrane antigen in an immunogenic form.
    J Gen Virol. 1983 Feb;64 (Pt 2):455-60 PMID: 6300296
  58. Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus.
    J Virol. 1976 Mar;17(3):935-49 PMID: 176452
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.6.1796-1807.1987
Published
1987-06-00
Pages
1796-807
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254182
Subset
IM
Grants
NCI NIH HHS · CA 17281 · United States
NCI NIH HHS · CA 19264 · United States
NIGMS NIH HHS · GM07281 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.6.1796-1807.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2