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PMID: 3035501 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Dual tissue-specific expression of apo-AII is directed by an upstream enhancer.

Nucleic acids research ·Vol. 15 ·No. 9 ·1987-05-11 ·Pages 3801-21

Shelley CS, Baralle FE

Abstract

Apolipoprotein-AII (apo-AII) is one of a family of evolutionarily related proteins which play a crucial role in lipid transport and metabolism. The serum levels of human apo-AII have been shown to be inversely correlated to the incidence of coronary heart disease and its expression to be limited to the liver and intestine. Here we demonstrate that this dual tissue-specificity involves DNA sequences located in a 259 bp region centred 782 bp upstream from the transcription initiation site. These sequences function in an orientation-independent manner and are absolutely required for transcription from the apo-AII promoter. The regulatory region contains sequences which are homologous to the apo-AI, beta-globin and immunoglobulin gene promoters and to the immunoglobulin heavy-chain enhancer.

MeSH Terms
Apolipoprotein A-II Apolipoproteins A/biosynthesis,genetics Carcinoma, Hepatocellular/pathology Cell Line Colonic Neoplasms/pathology Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator HeLa Cells/metabolism Humans Intestinal Mucosa/metabolism Liver/metabolism Liver Neoplasms Organ Specificity Recombinant Fusion Proteins/biosynthesis
Chemicals
Apolipoprotein A-II Apolipoproteins A Recombinant Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shelley C S
Baralle F E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.9.3801
Published
1987-05-11
Pages
3801-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340783
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.9.3801 PMC Full Text View journal details
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