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PMID: 31244561 Published · epublish English Journal Article

Differential motility parameters and identification of proteomic profiles of human sperm cryopreserved with cryostraw and cryovial.

Clinical proteomics ·Vol. 16 ·2019-00-00 ·Pages 24

Li S, Ao L, Yan Y, Jiang J, Chen B, Duan Y, Shen F, Chen J, Inglis B, Ni R, Ji W, Si W

Abstract

Although sperm cryopreservation has been widely used in human reproductive medicine as an integral infertility management in infertility clinics and for banking sperm in sperm banks, the freezing/thawing protocols are not optimal. The freezing and thawing processes result in changes at both structural and molecular levels, some even detrimental, in human sperm when compared with fresh sperm. The change of sperm proteins after cryopreservation may play negative roles for fertilization and early embryo development. Conventionally, cryostraws (CS) and cryovials (CV) are the most widely used cryopreservation carriers (CPCs) for human sperm cryopreservation accompanied with the use of egg yolk free commercial media. However, the influence of cryopreservation on the proteomic profile of human sperm preserved with the two CPCs is unknown. Therefore the purpose of the present study was to compare the frozen-thawed motility, investigate the proteomic profile of human sperm cryopreserved with the two types of CPCs, and identify the susceptible proteins that play key roles for sperm function and fertility. The present study compared the cryosurvival of human sperm frozen with the two different CPCs and identified the sperm proteomic changes by using the isobaric tags for relative and absolute quantification labeling technique coupled with 2D LC-MS/MS analysis after freezing and thawing. Our results indicated that sperm cryopreserved with CV showed higher values for percentage of motile sperm and forward activity rate than those with CS. Compared to fresh sperm, 434 and 432 proteins were differentially identified in human sperm cryopreserved with CS and CV, respectively. The proteomic profiles of human sperm are greatly affected by cryopreservation with either type of CPC. GO analysis revealed that most of the differentially identified sperm proteins enriched in the extracellular membrane-bounded organelles, cytoplasm and cytosol. In addition, 106 susceptible proteins having known identities related to sperm functions were identified. In general, cryovial seems to be the preferred CPC for human sperm cryopreservation based on the post-thaw motility parameters and the effect on sperm proteomic profiles. These results are beneficial for the insight into the understanding of the cryoinjury mechanism of sperm and the development of human sperm cryopreservation strategies.

Keywords
Cryopreservation Cryostraw Cryovial Human sperm Proteomic profile iTRAQ
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Li Shanshan
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Ao Lei
Kunming Sino-UK Angel Women's & Children's Hospital, Kunming, Yunnan China.
Yan Yaping
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Jiang Jiang
3Department of Obstetrics, The First People's Hospital of Yunnan Province, Kunming, Yunnan China.
Chen Bingbing
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Duan Yanchao
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Shen Fei
Kunming Sino-UK Angel Women's & Children's Hospital, Kunming, Yunnan China.
Chen Jinbao
Kunming Sino-UK Angel Women's & Children's Hospital, Kunming, Yunnan China.
Inglis Briauna
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Ni Renmin
Kunming Sino-UK Angel Women's & Children's Hospital, Kunming, Yunnan China.
Ji Weizhi
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Si Wei ORCID
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan China.
Conflict of Interest

Competing interestsThe authors declare that they have no competing interests.

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Article Info
Journal
Clinical proteomics
Abbr.
Clin Proteomics
ISSN
1542-6416
DOI
10.1186/s12014-019-9244-2
Published
2019-00-00
Epub
2019-00-19
Pages
24
Language
English
Region
England
NLM ID
101184586
PMCID
PMC6582484
External Links
PubMed source DOI: 10.1186/s12014-019-9244-2 PMC Full Text View journal details
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