Home LiteratureArticle Details
PMID: 3325773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of the domain of Saccharomyces cerevisiae adenylate cyclase associated with the regulatory function of RAS products.

Molecular & general genetics : MGG ·Vol. 210 ·No. 2 ·1987-12-00 ·Pages 187-94

Uno I, Mitsuzawa H, Tanaka K, Oshima T, Ishikawa T

Abstract

Various truncated CYR1 genes of Saccharomyces cerevisiae were fused to efficient promoters and expressed in Escherichia coli and S. cerevisiae cells with or without the RAS genes. The catalytic domain of adenylate cyclase encoded by the 3'-terminal 1.3 kb region of the open reading frame of the CYR1 gene produced cyclic AMP, irrespective of the presence of RAS genes. The product of the 3'-terminal 2.1 kb region of CYR1 showed guanine nucleotide-dependent adenylate cyclase activity and produced a large amount of cAMP in the presence of the RAS gene. Thus, the domain encoded by the 0.8 kb region adjacent to the catalytic domain is associated with the regulatory function of the RAS products. The cyr1 RAS1 RAS2 cells carrying the 3'-terminal 1.3 kb region of CYR1 were unable to respond to environmental signals such as sulfur starvation and temperature shift, but the cyr1 cells carrying the 2.1 kb region and at least one RAS gene were able to respond to these signals. The environmental signals may be transferred to the adenylate cyclase system through the RAS products.

MeSH Terms
Adenylyl Cyclases/genetics Cell Cycle Escherichia coli/genetics Gene Expression Regulation Genes, Fungal Genes, Regulator Genes, ras Mutation Plasmids Saccharomyces cerevisiae/cytology,enzymology,genetics
Chemicals
Adenylyl Cyclases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Uno I
Institute of Applied Microbiology, University of Tokyo, Japan.
Mitsuzawa H
Tanaka K
Oshima T
Ishikawa T
References (36)
36 references, click to expand
  1. Expression and characterization of ras mRNAs from Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Nov;4(11):2298-305 PMID: 6392849
  2. Nucleotide sequence of two rasH related-genes isolated from the yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1984 Apr 25;12(8):3611-8 PMID: 6328429
  3. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.
    Methods Enzymol. 1983;100:468-500 PMID: 6225933
  4. DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase.
    Cell. 1985 Dec;43(2 Pt 1):493-505 PMID: 2934138
  5. Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation.
    Mol Cell Biol. 1987 Jan;7(1):244-50 PMID: 3031463
  6. Isolation of the gene encoding adenylate cyclase in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5060-3 PMID: 2991907
  7. Identification of the structural gene and nonsense alleles for adenylate cyclase in Saccharomyces cerevisiae.
    J Bacteriol. 1984 Jan;157(1):277-82 PMID: 6360999
  8. Reconstitution of the GTP-dependent adenylate cyclase from products of the yeast CYR1 and RAS2 genes in Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):7855-9 PMID: 2999779
  9. Genetic analysis of the role of cAMP in yeast.
    Yeast. 1985 Sep;1(1):15-24 PMID: 2851898
  10. Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):137-44 PMID: 327270
  11. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  12. The cya locus of Escherichia coli K12: organization and gene products.
    Mol Gen Genet. 1982;188(3):465-71 PMID: 6298576
  13. Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins.
    Cell. 1985 Jul;41(3):763-9 PMID: 3891097
  14. Molecular cloning of the tsm0185 gene responsible for adenylate cyclase activity in Saccharomyces cerevisiae.
    Ann Microbiol (Paris). 1984 May-Jun;135A(3):343-51 PMID: 6380368
  15. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
    Cell. 1984 Mar;36(3):607-12 PMID: 6365329
  16. Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes.
    J Cell Biol. 1984 Apr;98 (4):1185-93 PMID: 6371018
  17. Centromeric DNA from Saccharomyces cerevisiae.
    J Mol Biol. 1982 Jun 25;158(2):157-90 PMID: 6750136
  18. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  19. A product of yeast RAS2 gene is a guanine nucleotide binding protein.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6924-8 PMID: 6438624
  20. The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae.
    J Biol Chem. 1979 Oct 10;254(19):9839-45 PMID: 385592
  21. A guanine nucleotide-sensitive adenylate cyclase in the yeast Saccharomyces cerevisiae.
    J Biol Chem. 1983 Jul 10;258(13):7911-4 PMID: 6345532
  22. Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PHO4.
    Nucleic Acids Res. 1986 Apr 11;14(7):3059-73 PMID: 3008105
  23. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  24. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2355-9 PMID: 6285379
  25. In yeast, RAS proteins are controlling elements of adenylate cyclase.
    Cell. 1985 Jan;40(1):27-36 PMID: 2981630
  26. Yeast adenylate cyclase catalytic domain is carboxy terminal.
    Curr Genet. 1986;10(5):343-52 PMID: 3327602
  27. ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae.
    Nature. 1983 Dec 15-21;306(5944):707-9 PMID: 6318116
  28. Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.
    Gene. 1983 Apr;22(1):103-13 PMID: 6305768
  29. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  30. Yeast and mammalian ras proteins have conserved biochemical properties.
    Nature. 1985 Feb 21-27;313(6004):700-3 PMID: 3919305
  31. Control of cell division in Saccharomyces cerevisiae mutants defective in adenylate cyclase and cAMP-dependent protein kinase.
    Exp Cell Res. 1983 Jun;146(1):151-61 PMID: 6305691
  32. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  33. A protein binding assay for adenosine 3':5'-cyclic monophosphate.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12 PMID: 4318781
  34. Translational efficiency of the Escherichia coli adenylate cyclase gene: mutating the UUG initiation codon to GUG or AUG results in increased gene expression.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5656-60 PMID: 3898067
  35. Guanine nucleotide activation of, and competition between, RAS proteins from Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Jun;7(6):2128-33 PMID: 3299060
  36. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00325683
Published
1987-12-00
Pages
187-94
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00325683
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2