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PMID: 3336367 Published · ppublish English Journal Article

DNase I- and micrococcal nuclease-hypersensitive sites in the human apolipoprotein B gene are tissue specific.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 71-80

Levy-Wilson B, Fortier C, Blackhart BD, McCarthy BJ

Abstract

We have mapped the DNase I- and micrococcal nuclease-hypersensitive sites present in the 5' end of the human apolipoprotein B (apo-B) gene in nuclei from cells expressing or not expressing the gene. Four DNase I-hypersensitive sites were found in nuclei from liver-derived HepG2 cells and intestine-derived CaCo-2 cells, which express the apo-B gene, but not in HeLa cells, which do not. These sites are located near positions -120, -440, -700, and +760 base pairs relative to the transcriptional start site. Undifferentiated CaCo-2 cells exhibited another site, near position -540. Six micrococcal nuclease-hypersensitive sites were found in nuclei from HepG2 and CaCo-2 cells, but not in HeLa cells or free DNA. These sites are located near positions -120, -390, -530, -700, -850, and +210. HepG2 cells exhibited another site, near position +460. Comparison of the DNA sequence of the 5' flanking regions of the human and mouse apo-B genes revealed a high degree of evolutionary conservation of short stretches of sequences in the immediate vicinity of each of the DNase I- and most of the micrococcal nuclease-hypersensitive sites.

MeSH Terms
Apolipoproteins B/genetics Base Sequence Cell Line Chromatin/ultrastructure Deoxyribonuclease I Gene Expression Regulation Humans Micrococcal Nuclease Nucleosomes/ultrastructure Promoter Regions, Genetic RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid
Chemicals
Apolipoproteins B Chromatin Nucleosomes RNA, Messenger Deoxyribonuclease I Micrococcal Nuclease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Levy-Wilson B
Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140-0608.
Fortier C
Blackhart B D
McCarthy B J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.71-80.1988
Published
1988-01-00
Pages
71-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363081
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.1.71-80.1988 PMC Full Text View journal details
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