Home LiteratureArticle Details
PMID: 3499313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Additive effects of interleukin 1 and tumour necrosis factor-alpha on the accumulation of the three granulocyte and macrophage colony-stimulating factor mRNAs in human endothelial cells.

The EMBO journal ·Vol. 6 ·No. 8 ·1987-08-00 ·Pages 2261-5

Seelentag WK, Mermod JJ, Montesano R, Vassalli P

Abstract

The control of haematopoietic colony-stimulating factors (CSF) gene expression by interleukin 1 (IL-1) and tumour necrosis factor alpha (TNF-alpha) in cultured endothelial cells was studied by RNA hybridization and nuclear gene transcription. Both IL-1 and TNF-alpha induced, with somewhat different kinetics, a slow but marked accumulation of granulocyte-macrophage (GM)- and granulocyte (G)-CSF mRNAs in endothelial cells; macrophage (M)-CSF mRNA increased more rapidly but more moderately. Simultaneous treatment with maximally stimulating concentrations of both IL-1 and TNF-alpha had an additive effect on the accumulation of the three mRNAs, suggesting that both mediators act via independent pathways. The mechanism of CSF mRNA accumulation in endothelial cells was explored by nuclear run-on experiments, which showed that both IL-1 and TNF-alpha increase GM-CSF, G-CSF and M-CSF gene transcription to varying degrees.

MeSH Terms
Cells, Cultured Endothelium/drug effects,metabolism Female Genes/drug effects Humans Interleukin-1/pharmacology Interleukin-3/genetics Lymphotoxin-alpha/pharmacology RNA, Messenger/drug effects,genetics Recombinant Proteins/pharmacology Transcription, Genetic/drug effects Tumor Necrosis Factor-alpha/pharmacology Umbilical Veins
Chemicals
Interleukin-1 Interleukin-3 Lymphotoxin-alpha RNA, Messenger Recombinant Proteins Tumor Necrosis Factor-alpha
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Seelentag W K
Department of Pathology, University of Geneva, Switzerland.
Mermod J J
Montesano R
Vassalli P
References (42)
42 references, click to expand
  1. Production of lymphocyte-activating factor (Interleukin 1) by macrophages activated with colony-stimulating factors.
    J Immunol. 1980 Sep;125(3):1302-5 PMID: 6774016
  2. Phorbol esters induce angiogenesis in vitro from large-vessel endothelial cells.
    J Cell Physiol. 1987 Feb;130(2):284-91 PMID: 2434516
  3. Autoinduction of differentiation in WEHI-3B leukemia cells.
    Int J Cancer. 1982 Dec 15;30(6):773-80 PMID: 6984432
  4. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon.
    J Exp Med. 1983 Apr 1;157(4):1339-53 PMID: 6403654
  5. A monokine regulates colony-stimulating activity production by vascular endothelial cells.
    Blood. 1983 Sep;62(3):663-8 PMID: 6603879
  6. Role of endothelial cells in human hematopoiesis: modulation of mixed colony growth in vitro.
    Blood. 1984 Mar;63(3):553-8 PMID: 6607755
  7. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells.
    J Exp Med. 1984 Aug 1;160(2):618-23 PMID: 6332168
  8. Leukocyte interleukins induce cultured endothelial cells to produce a highly organized, glycosaminoglycan-rich pericellular matrix.
    J Cell Biol. 1984 Nov;99(5):1706-15 PMID: 6333426
  9. Interleukin-1 and the pathogenesis of the acute-phase response.
    N Engl J Med. 1984 Nov 29;311(22):1413-8 PMID: 6208485
  10. Human endothelial cell cultures: phenotypic modulation by leukocyte interleukins.
    J Cell Physiol. 1985 Mar;122(3):424-34 PMID: 3871441
  11. Monocyte-derived recruiting activity: kinetics of production and effects of endotoxin.
    Blood. 1985 Mar;65(3):689-95 PMID: 3871645
  12. Identification of the human analogue of a regulator that induces differentiation in murine leukaemic cells.
    Nature. 1985 Apr 18-24;314(6012):625-8 PMID: 2986009
  13. Expression of I-region-associated antigen (Ia) and interleukin 1 by subcultured human endothelial cells.
    Cell Immunol. 1985 Jun;93(1):91-104 PMID: 3873291
  14. Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6250-4 PMID: 3898082
  15. Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity.
    J Clin Invest. 1985 Sep;76(3):1286-90 PMID: 3876355
  16. Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).
    Science. 1985 Oct 18;230(4723):291-6 PMID: 2996129
  17. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines.
    J Clin Invest. 1985 Nov;76(5):2003-11 PMID: 3877078
  18. Interleukin 1 increases the binding of human B and T lymphocytes to endothelial cell monolayers.
    J Immunol. 1986 Jan;136(1):203-7 PMID: 3079607
  19. Cultured human vascular endothelial cells acquire adhesiveness for neutrophils after stimulation with interleukin 1, endotoxin, and tumor-promoting phorbol diesters.
    J Immunol. 1986 Jan;136(2):649-54 PMID: 2416819
  20. Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8667-71 PMID: 3866246
  21. Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):446-50 PMID: 3455781
  22. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.
    Nature. 1986 Jan 30-Feb 5;319(6052):415-8 PMID: 3484805
  23. Production of interleukin 1 by human endothelial cells.
    J Immunol. 1986 Apr 1;136(7):2486-91 PMID: 3485151
  24. Induction of macrophage tumoricidal activity by granulocyte-macrophage colony-stimulating factor.
    Science. 1986 Apr 25;232(4749):506-8 PMID: 3083507
  25. Interleukin 1 and lipopolysaccharide induce an inhibitor of tissue-type plasminogen activator in vivo and in cultured endothelial cells.
    J Exp Med. 1986 May 1;163(5):1260-6 PMID: 3084701
  26. Cachectin and tumour necrosis factor as two sides of the same biological coin.
    Nature. 1986 Apr 17-23;320(6063):584-8 PMID: 3010124
  27. Interleukin 1 induces endothelial cell procoagulant while suppressing cell-surface anticoagulant activity.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3460-4 PMID: 3486418
  28. Interleukin 1 stimulates fibroblasts to produce granulocyte-macrophage colony-stimulating activity and prostaglandin E2.
    J Clin Invest. 1986 Jun;77(6):1857-63 PMID: 3486886
  29. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1.
    J Exp Med. 1986 Jun 1;163(6):1363-75 PMID: 3011946
  30. Interleukin 1 induces endothelial cell synthesis of plasminogen activator inhibitor.
    J Exp Med. 1986 Jun 1;163(6):1595-600 PMID: 3086483
  31. Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4533-7 PMID: 3487091
  32. Regulation of the fibrinolytic system of cultured human vascular endothelium by interleukin 1.
    J Clin Invest. 1986 Aug;78(2):587-91 PMID: 3090105
  33. UV crosslinking of RNA to nylon membrane enhances hybridization signals.
    Mol Biol Rep. 1986;11(2):107-15 PMID: 2426570
  34. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells.
    Am J Pathol. 1986 Aug;124(2):179-85 PMID: 3526909
  35. Recombinant human TNF induces production of granulocyte-monocyte colony-stimulating factor.
    Nature. 1986 Sep 4-10;323(6083):79-82 PMID: 3489188
  36. Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7467-71 PMID: 3489939
  37. The role of interleukin-1 in neutrophil leukocyte emigration induced by endotoxin.
    Am J Pathol. 1986 Sep;124(3):367-72 PMID: 3532812
  38. Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells.
    J Clin Invest. 1986 Nov;78(5):1316-23 PMID: 3490494
  39. Gamma interferon enhances macrophage transcription of the tumor necrosis factor/cachectin, interleukin 1, and urokinase genes, which are controlled by short-lived repressors.
    J Exp Med. 1986 Dec 1;164(6):2113-8 PMID: 3097240
  40. Interleukin 1 induces cultured human endothelial cell production of granulocyte-macrophage colony-stimulating factor.
    J Clin Invest. 1987 Jan;79(1):48-51 PMID: 3491839
  41. Phagocytosis and inflammatory stimuli induce GM-CSF mRNA in macrophages through posttranscriptional regulation.
    Cell. 1987 Feb 27;48(4):671-9 PMID: 3102073
  42. Vascular endothelium as a regulator of granulopoiesis: production of colony-stimulating activity by cultured human endothelial cells.
    Blood. 1980 Dec;56(6):1060-7 PMID: 6969095
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-08-00
Pages
2261-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553627
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2