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PMID: 353517 Published · ppublish English Journal Article

Detection of specific DNA sequences in yeast by colony hybridization.

Molecular & general genetics : MGG ·Vol. 161 ·No. 3 ·1978-05-31 ·Pages 311-5

Blanc H, Dujon B, Guerineau M, Slonimski PP

Abstract

A procedure is described for the detection of specific DNA sequences in Saccharomyces cerevisiae. This method allows a rapid screening of a large number of yeast colonies. The yeast cells of each colony, grown on nitrocellulose filters, are converted, in situ, to protoplasts by snail enzyme, and are then lysed and their DNAs are denatured and fixed on the filter. The presence of the specific DNA sequence is detected directly on the filter by hybridization with a radioactive cRNA. We have used successfully this technique to detect the presence or the absence of specific mt DNA sequences in p+, p- and p0 strains, and to detect the presence or the absence of the 2 mum DNA sequences in different strains.

MeSH Terms
Base Sequence Clone Cells DNA, Circular/analysis DNA, Mitochondrial/analysis Escherichia coli/genetics Nucleic Acid Hybridization Phosphorus Radioisotopes RNA, Bacterial/metabolism Saccharomyces cerevisiae/genetics
Chemicals
DNA, Circular DNA, Mitochondrial Phosphorus Radioisotopes RNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blanc H
Dujon B
Guerineau M
Slonimski P P
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331006
Published
1978-05-31
Pages
311-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331006
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