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PMID: 3541901 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12.

The Biochemical journal ·Vol. 237 ·No. 2 ·1986-07-15 ·Pages 547-57

Woods SA, Miles JS, Roberts RE, Guest JR

Abstract

The nucleotide sequences of two segments of DNA (2250 and 2921 base-pairs) containing the functionally related fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12 were determined. The fumC structural gene comprises 1398 base-pairs (466 codons, excluding the initiation codon), and it encodes a polypeptide of Mr 50353 that resembles the fumarases of Bacillus subtilis 168 (citG-gene product), rat liver and pig heart. The fumC gene starts 140 base-pairs downstream of the structurally-unrelated fumA gene, but there is no evidence that both genes form part of the same operon. The aspA structural gene comprises 1431 base-pairs (477 codons excluding the initiation codon), and it encodes a polypeptide of Mr 52190, similar to that predicted from maxicell studies and for the enzyme from E. coli W. Remarkable homologies were found between the primary structures of the fumarase (fumC and citG) and aspartase (aspA) genes and their products, suggesting close structural and evolutionary relationships.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Ammonia-Lyases/genetics Aspartate Ammonia-Lyase/genetics Base Sequence Cloning, Molecular Codon/genetics DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Fumarate Hydratase/genetics Genes
Chemicals
Amino Acids Codon DNA, Bacterial Fumarate Hydratase Ammonia-Lyases Aspartate Ammonia-Lyase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Woods S A
Miles J S
Roberts R E
Guest J R
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2370547
Published
1986-07-15
Pages
547-57
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147019
Subset
IM
Databases
GENBANK
X04065, X04066
External Links
PubMed source DOI: 10.1042/bj2370547 PMC Full Text View journal details
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