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PMID: 366614 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 12 ·1978-12-00 ·Pages 6177-81

Harris JD, Heilig JS, Martinez II, Calendar R, Isaksson LA

Abstract

A gene affecting the sigma subunit of DNA-dependent RNA polymerase is tightly linked to dnaG at 66 min on the Escherichia coli chromosome. In order to create an easily selectable marker in this region, we inserted transposon-10, which carries a gene determining resistance to tetracycline (tet) near 66 min, and the order tolC-dnaG-sigma-tet was determined. We used frequency of contransduction with tet as a criterion to screen a collection of spontaneous temperature-sensitive Escherichia coli mutants that might affect the sigma subunit. One such mutant was found to map at the sigma locus. The sigma subunit isolated from this mutant is unstable at 46 degrees C in vitro and has an altered electrophoretic mobility. The temperature sensitivity of RNA synthesis in this mutant indicates that most transcription in E. coli is sigma dependent.

MeSH Terms
Chromosome Mapping DNA-Directed RNA Polymerases/genetics Escherichia coli/enzymology,genetics Genes Hot Temperature Isoelectric Point Mutation RNA, Bacterial/biosynthesis Sigma Factor/genetics Transcription Factors/genetics Translocation, Genetic
Chemicals
RNA, Bacterial Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harris J D
Heilig J S
Martinez I I
Calendar R
Isaksson L A
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.12.6177
Published
1978-12-00
Pages
6177-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393142
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.12.6177 PMC Full Text View journal details
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