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PMID: 3670289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Glucocorticoids selectively inhibit translation of ribosomal protein mRNAs in P1798 lymphosarcoma cells.

Molecular and cellular biology ·Vol. 7 ·No. 8 ·1987-08-00 ·Pages 2691-9

Meyuhas O, Thompson EA, Perry RP

Abstract

When P1798 murine lymphosarcoma cells are exposed to 10(-7) M dexamethasone, there is a dramatic inhibition of rRNA synthesis, which is completely reversible when the hormone is withdrawn. In the present experiments we examined whether dexamethasone treatment causes any alteration in the accumulation or utilization of mRNAs that encode ribosomal proteins (rp mRNAs). No effect on the accumulation of six different rp mRNAs was detected. However, the translation of five of six rp mRNAs was selectively inhibited in the presence of the hormone, as judged by a substantial decrease in ribosomal loading. Normal translation of rp mRNA was resumed within a few hours after hormone withdrawal. In untreated or fully recovered cells, the distribution of rp mRNAs between polyribosomes and free ribonucleoprotein is distinctly bimodal, suggesting that rp mRNAs are subject to a particular form of translational control in which they are either translationally inactive or fully loaded with ribosomes. A possible relationship between this mode of translational control and the selective suppression of rp mRNA translation by glucocorticoids is discussed.

MeSH Terms
Animals Cell Line DNA, Ribosomal/drug effects,genetics Dexamethasone/pharmacology Kinetics Lymphoma, Non-Hodgkin/genetics,metabolism Mice Polyribosomes/metabolism Protein Biosynthesis/drug effects RNA, Messenger/drug effects,genetics Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Messenger Ribosomal Proteins Dexamethasone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meyuhas O
Institute of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Thompson E A
Perry R P
References (59)
59 references, click to expand
  1. Regulation of ribosomal protein mRNA content and translation in growth-stimulated mouse fibroblasts.
    Mol Cell Biol. 1982 Jun;2(6):685-93 PMID: 14582163
  2. Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
    Methods Enzymol. 1983;100:255-66 PMID: 6194404
  3. Persistent cytoplasmic synthesis of ribosomal proteins during the selective inhibition of ribosomal RNA synthesis.
    Nat New Biol. 1971 Jan 20;229(3):75-80 PMID: 5280340
  4. The cocoonase zymogen cells of silk moths: a model of terminal cell differentiation for specific protein synthesis.
    Curr Top Dev Biol. 1972;7:125-91 PMID: 4643798
  5. Model for the regulation of mRNA translation applied to haemoglobin synthesis.
    Nature. 1974 Oct 4;251(5474):385-8 PMID: 4421673
  6. In the absence of ribosomal RNA synthesis, the ribosomal proteins of HeLa cells are synthesized normally and degraded rapidly.
    J Mol Biol. 1977 Sep 25;115(3):315-33 PMID: 592369
  7. Regulation of translation of ovalbumin messenger RNA by estrogens and progesterone in oviduct of withdrawn chicks.
    Eur J Biochem. 1978 Sep 15;90(1):51-8 PMID: 710420
  8. Control of globin gene expression by steroid hormones in differentiating Friend leukemia cells.
    Cell. 1978 Oct;15(2):447-53 PMID: 281261
  9. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  10. The structure and function of eukaryotic ribosomes.
    Annu Rev Biochem. 1979;48:719-54 PMID: 382996
  11. Immunoglobulin messenger RNAs in murine cell lines that have characteristics of immature B lymphocytes.
    Cell. 1979 Dec;18(4):1333-9 PMID: 117905
  12. Properties of a cell-culture line derived from lymphosarcoma P1798.
    Mol Cell Endocrinol. 1980 Feb;17(2):95-102 PMID: 6892700
  13. Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation.
    Biochemistry. 1980 Apr 15;19(8):1662-9 PMID: 6990980
  14. Construction and identification of cDNA clones for mouse ribosomal proteins: application for the study of r-protein gene expression.
    Gene. 1980 Jul;10(2):113-29 PMID: 6993285
  15. Unco-ordinate regulation of ribosomal RNA and ribosomal protein synthesis during L6E9 myoblast differentiation.
    J Mol Biol. 1980 Sep 15;142(2):145-59 PMID: 7441756
  16. Mouse actin messenger RNAs. Construction and characterization of a recombinant plasmid molecule containing a complementary DNA transcript of mouse alpha-actin mRNA.
    J Biol Chem. 1981 Jan 25;256(2):1008-14 PMID: 6256358
  17. Lodish model and regulation of ribosomal protein synthesis by insulin-deficient chick embryo fibroblasts.
    Biochemistry. 1981 Apr 28;20(9):2550-8 PMID: 7016179
  18. Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.
    Cell. 1981 Nov;27(1 Pt 2):165-74 PMID: 7326749
  19. Coordinate regulation of ribosomal protein mRNA level in regenerating rat liver. Study with the corresponding mouse cloned cDNAs.
    Nucleic Acids Res. 1982 Feb 11;10(3):789-801 PMID: 6121318
  20. Coordinate control of syntheses of ribosomal ribonucleic acid and ribosomal proteins during nutritional shift-up in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Nov;1(11):1007-15 PMID: 7050661
  21. Yeast use translational control to compensate for extra copies of a ribosomal protein gene.
    Cell. 1982 Jun;29(2):347-55 PMID: 7116444
  22. Structure and organization of a mammalian 5 S gene cluster.
    J Biol Chem. 1982 Oct 10;257(19):11706-11 PMID: 6896875
  23. Expression of ribosomal-protein genes in Xenopus laevis development.
    Cell. 1982 Aug;30(1):163-71 PMID: 6889922
  24. Glucose-6-phosphate dehydrogenase. Translational regulation of synthesis and regulation of processing of the enzyme in the uterus by estradiol.
    Biochim Biophys Acta. 1983 Mar 10;739(2):148-57 PMID: 6824671
  25. The nucleotide sequence of the rat cytoplasmic beta-actin gene.
    Nucleic Acids Res. 1983 Mar 25;11(6):1759-71 PMID: 6300777
  26. Mild temperature shock alters the transcription of a discrete class of Saccharomyces cerevisiae genes.
    Mol Cell Biol. 1983 Mar;3(3):457-65 PMID: 6341818
  27. Hormonal regulation of transcription of rDNA. Inhibition of transcription during glucocorticoid-mediated inhibition of proliferation of lymphosarcoma P1798 cells in culture.
    J Biol Chem. 1983 Aug 25;258(16):9768-73 PMID: 6688417
  28. Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA.
    Mol Cell Biol. 1983 Jul;3(7):1317-32 PMID: 6412070
  29. Glucocorticoids inhibit the coordinated translation of alpha- and beta-globin mRNAs in Friend erythroleukemia cells.
    Arch Biochem Biophys. 1983 Dec;227(2):542-51 PMID: 6582803
  30. Glucocorticoid inhibition of initiation of transcription of the DNA encoding rRNA (rDNA) in lymphosarcoma P1798 cells.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):718-21 PMID: 6322167
  31. Complete nucleotide sequence of the messenger RNA coding for chicken muscle glyceraldehyde-3-phosphate dehydrogenase.
    Biochem Biophys Res Commun. 1984 Feb 14;118(3):767-73 PMID: 6322764
  32. The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.
    EMBO J. 1984 Jan;3(1):29-34 PMID: 6200323
  33. Relationship between rates of synthesis and intracellular distribution of ribosomal proteins during oogenesis in the mouse.
    Dev Biol. 1984 Apr;102(2):525-30 PMID: 6706014
  34. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147-51 PMID: 6326107
  35. The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene.
    Cell. 1984 Jun;37(2):457-68 PMID: 6327068
  36. Molecular cloning and biosynthetic regulation of cry1 gene of Saccharomyces cerevisiae.
    Mol Gen Genet. 1984;195(3):500-6 PMID: 6088947
  37. Regulation of the synthesis of ribosomes and ribosomal components.
    Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
  38. Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family.
    Mol Cell Biol. 1984 Nov;4(11):2518-28 PMID: 6513928
  39. Expression of two Xenopus laevis ribosomal protein genes in injected frog oocytes. A specific splicing block interferes with the L1 RNA maturation.
    J Mol Biol. 1984 Dec 25;180(4):987-1005 PMID: 6084725
  40. The induction of ribosome biosynthesis in a nonmitotic secretory tissue.
    J Biol Chem. 1985 Jun 25;260(12):7645-50 PMID: 2581968
  41. Hormonal regulation of transcription of rDNA: glucocorticoid effects upon initiation and elongation in vitro.
    Nucleic Acids Res. 1985 May 10;13(9):3357-69 PMID: 4000974
  42. Ribosomal protein production in normal and anucleolate Xenopus embryos: regulation at the posttranscriptional and translational levels.
    Cell. 1985 Aug;42(1):317-23 PMID: 4016954
  43. Differentiation of rat myoblasts. Regulation of turnover of ribosomal proteins and their mRNAs.
    Eur J Biochem. 1985 Jul 15;150(2):255-63 PMID: 4018082
  44. Saccharomyces cerevisiae coordinates accumulation of yeast ribosomal proteins by modulating mRNA splicing, translational initiation, and protein turnover.
    Mol Cell Biol. 1985 Jun;5(6):1512-21 PMID: 3897837
  45. Coordinate expression of ribosomal protein genes during Xenopus development.
    Dev Biol. 1985 Oct;111(2):488-98 PMID: 3840103
  46. Steroid receptor regulated transcription of specific genes and gene networks.
    Annu Rev Genet. 1985;19:209-52 PMID: 3909942
  47. Translational regulation of mRNAs for ribosomal proteins during early Drosophila development.
    Biochemistry. 1985 Oct 8;24(21):5798-803 PMID: 3936537
  48. Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns.
    EMBO J. 1985 Dec 16;4(13A):3483-8 PMID: 3841512
  49. Autogenous regulation of splicing of the transcript of a yeast ribosomal protein gene.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5854-7 PMID: 3526341
  50. Effect of RP51 gene dosage alterations on ribosome synthesis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Dec;5(12):3429-35 PMID: 3915776
  51. Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.
    Mol Cell Biol. 1985 Dec;5(12):3560-76 PMID: 3915781
  52. Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster.
    Mol Cell Biol. 1985 Dec;5(12):3583-92 PMID: 3939320
  53. Transcriptional regulation of ribosomal proteins during a nutritional upshift in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jul;6(7):2429-35 PMID: 3023933
  54. The primary structure of rat ribosomal protein L19. A determination from the sequence of nucleotides in a cDNA and from the sequence of amino acids in the protein.
    J Biol Chem. 1987 Jan 25;262(3):1111-5 PMID: 3542997
  55. Transcriptional and translational regulation of ribosomal protein formation during mouse myoblast differentiation.
    J Biol Chem. 1987 Apr 5;262(10):4868-75 PMID: 3558374
  56. The cellular level of yeast ribosomal protein L25 is controlled principally by rapid degradation of excess protein.
    Curr Genet. 1986;10(10):733-9 PMID: 3329033
  57. Messenger RNA species partially in a repressed state in mouse sarcoma ascites cells.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5876-80 PMID: 6964392
  58. Nucleotide sequence and expression of human chromosome 21-encoded superoxide dismutase mRNA.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5465-9 PMID: 6577438
  59. The second Sir Hans Krebs Lecture. Informosomes.
    Eur J Biochem. 1969 Aug;10(1):20-35 PMID: 4899460
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.8.2691-2699.1987
Published
1987-08-00
Pages
2691-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367885
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.8.2691-2699.1987 PMC Full Text View journal details
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