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PMID: 3926815 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Competitive, enzyme-linked immunosorbent assay for toxic shock syndrome toxin 1.

Journal of clinical microbiology ·Vol. 22 ·No. 1 ·1985-07-00 ·Pages 26-31

Parsonnet J, Mills JT, Gillis ZA, Pier GB

Abstract

We developed a competitive, enzyme-linked immunosorbent assay for the quantitation of toxic shock syndrome toxin 1 (TSST-1). Polyvalent immunoglobulin G from immunized rabbits was used as the capture antibody, and alkaline phosphatase conjugated to purified toxin served as the indicator enzyme. A standard curve was generated with each experiment, from which the concentration of toxin in culture supernatants was extrapolated. The assay was useful for determining toxin concentrations of 0.03 to 0.5 micrograms/ml, which is a substantial, practical improvement over immunodiffusion methods. Staphylococcal enterotoxins A through E were not significantly cross-reactive in the assay, and staphylococcal protein A did not interfere with quantitation of TSST-1. By testing a variety of staphylococcal strains, we found 100% concordance between toxin determinations made with our assay and those made by the investigators from whom the strains were obtained. The competitive, enzyme-linked immunosorbent assay is a highly reproducible, inexpensive means of determining TSST-1 concentrations and may have broad applicability in the field of toxic shock research.

MeSH Terms
Alkaline Phosphatase Animals Bacterial Toxins Cross Reactions Enterotoxins/analysis,immunology Enzyme-Linked Immunosorbent Assay Immunodiffusion Rabbits Species Specificity Staphylococcal Protein A Staphylococcus aureus Superantigens
Chemicals
Bacterial Toxins Enterotoxins Staphylococcal Protein A Superantigens enterotoxin F, Staphylococcal Alkaline Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parsonnet J
Mills J T
Gillis Z A
Pier G B
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25 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.22.1.26-31.1985
Published
1985-07-00
Pages
26-31
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC268314
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.22.1.26-31.1985 PMC Full Text View journal details
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