Specific alteration of the 30S ribosomal subunits of Bacillus subtilis during sporulation.

Journal of bacteriology ·Vol. 131 ·No. 3 ·1977-09-00 ·Pages 866-71

Guha S, Szulmajster J

Abstract

Active 30S ribosomal subunits were isolated from vegetative and sporulating cells of Bacillus subtilis. Both subunits were able to function in polyuridylic acid of phage phie messenger ribonucleic acid-dependent protein synthesis in vitro. The sporulation 30S subunits were highly active in polyuridylic acid-dependent polyphenylalanine synthesis but showed a reduced activity in the presence of natural messenger ribonucleic acid as compared with their vegetative counter-parts. The reduced activity was independent of the source of 50S particles and initiation factors (vegetative or sporulation). The alteration of the 30S sporulation subunits appears to be related to the sporulation process, since the same subunits isolated from stationary-phase cells of an asporogenic mutant did not show any impairment in protein synthesis in vitro.

MeSH Terms

Bacillus subtilis/growth & development,metabolism Bacterial Proteins/biosynthesis Coliphages/metabolism Peptide Initiation Factors Phenylalanine/metabolism Poly U/metabolism RNA, Messenger/metabolism RNA, Viral/metabolism Ribosomal Proteins/biosynthesis Ribosomes/metabolism Spores, Bacterial/growth & development,metabolism Subcellular Fractions

Chemicals

Bacterial Proteins Peptide Initiation Factors RNA, Messenger RNA, Viral Ribosomal Proteins Poly U Phenylalanine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Guha S

Szulmajster J

References (22)

22 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.131.3.866-871.1977

Published

1977-09-00

Pages

866-71

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC235542

Subset

External Links

PubMed source DOI: 10.1128/jb.131.3.866-871.1977 PMC Full Text View journal details

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Specific alteration of the 30S ribosomal subunits of Bacillus subtilis during sporulation.

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